RRC ID 46787
Author Takayama K, Kaneko H, Kataoka K, Kimoto R, Hwang SJ, Ye F, Nagasaka Y, Tsunekawa T, Matsuura T, Nonobe N, Ito Y, Terasaki H.
Title Nuclear Factor (Erythroid-Derived)-Related Factor 2-Associated Retinal Pigment Epithelial Cell Protection under Blue Light-Induced Oxidative Stress.
Journal Oxid Med Cell Longev
Abstract Purpose. It is a matter of increasing concern that exposure to light-emitting diodes (LED), particularly blue light (BL), damages retinal cells. This study aimed to investigate the retinal pigment epithelium (RPE) damage caused by BL and to elucidate the role of nuclear factor (erythroid-derived)-related factor 2 (Nrf2) in the pathogenesis of BL-induced RPE damage. Methods. ARPE-19, a human RPE cell line, and mouse primary RPE cells from wild-type and Nrf2 knockout (Nrf2-/-) mice were cultured under blue LED exposure (intermediate wavelength, 450 nm). Cell death rate and reactive oxygen species (ROS) generation were measured. TUNEL staining was performed to detect apoptosis. Real-time polymerase chain reaction was performed on NRF2 mRNA, and western blotting was performed to detect Nrf2 proteins in the nucleus or cytoplasm of RPE cells. Results. BL exposure increased cell death rate and ROS generation in ARPE-19 cells in a time-dependent manner; cell death was caused by apoptosis. Moreover, BL exposure induced NRF2 mRNA upregulation and Nrf2 nuclear translocation in RPE. Cell death rate was significantly higher in RPE cells from Nrf2-/- mice than from wild-type mice. Conclusions. The Nrf2 pathway plays an important role in protecting RPE cells against BL-induced oxidative stress.
Volume 2016
Pages 8694641
Published 2016-1-1
DOI 10.1155/2016/8694641
PMID 27774118
PMC PMC5059614
MeSH Active Transport, Cell Nucleus Animals Apoptosis / radiation effects Blotting, Western Cell Line Genotype Humans Light* Mice, Knockout NF-E2-Related Factor 2 / deficiency NF-E2-Related Factor 2 / genetics NF-E2-Related Factor 2 / metabolism* Oxidative Stress / radiation effects* Phenotype Reactive Oxygen Species / metabolism Real-Time Polymerase Chain Reaction Retinal Pigment Epithelium / metabolism Retinal Pigment Epithelium / pathology Retinal Pigment Epithelium / radiation effects* Reverse Transcriptase Polymerase Chain Reaction Time Factors Up-Regulation
IF 5.076
Times Cited 12
Mice RBRC01390