| RRC ID |
47083
|
| 著者 |
Kato K, Dong B, Wada H, Tanaka-Matakatsu M, Yagi Y, Hayashi S.
|
| タイトル |
Microtubule-dependent balanced cell contraction and luminal-matrix modification accelerate epithelial tube fusion.
|
| ジャーナル |
Nat Commun
|
| Abstract |
Connection of tubules into larger networks is the key process for the development of circulatory systems. In Drosophila development, tip cells of the tracheal system lead the migration of each branch and connect tubules by adhering to each other and simultaneously changing into a torus-shape. We show that as adhesion sites form between fusion cells, myosin and microtubules form polarized bundles that connect the new adhesion site to the cells' microtubule-organizing centres, and that E-cadherin and retrograde recycling endosomes are preferentially deposited at the new adhesion site. We demonstrate that microtubules help balancing tip cell contraction, which is driven by myosin, and is required for adhesion and tube fusion. We also show that retrograde recycling and directed secretion of a specific matrix protein into the fusion-cell interface promote fusion. We propose that microtubule bundles connecting these cell-cell interfaces coordinate cell contractility and apical secretion to facilitate tube fusion.
|
| 巻・号 |
7
|
| ページ |
11141
|
| 公開日 |
2016-4-12
|
| DOI |
10.1038/ncomms11141
|
| PII |
ncomms11141
|
| PMID |
27067650
|
| PMC |
PMC4832058
|
| MeSH |
Actins / metabolism
Animals
Biomechanical Phenomena
Cadherins / metabolism
Cell Fusion
Cell Movement
Drosophila melanogaster / cytology*
Drosophila melanogaster / metabolism
Epithelial Cells / cytology*
Extracellular Matrix / metabolism*
Green Fluorescent Proteins / metabolism
Intercellular Junctions / metabolism
Microtubules / metabolism*
Mutation / genetics
Myosins / metabolism
Phenotype
Protein Transport
Recombinant Fusion Proteins / metabolism
Trachea / cytology
|
| IF |
12.121
|
| 引用数 |
7
|
|
WOS 分野
|
CELL BIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
3327R-2
DGRC#119599
DGRC#119600
DGRC#119637
DGRC#119638 |
