RRC ID 47303
Author Takahagi K, Uehara-Yamaguchi Y, Yoshida T, Sakurai T, Shinozaki K, Mochida K, Saisho D.
Title Analysis of single nucleotide polymorphisms based on RNA sequencing data of diverse bio-geographical accessions in barley.
Journal Sci Rep
Abstract Barley is one of the founder crops of Old world agriculture and has become the fourth most important cereal worldwide. Information on genome-scale DNA polymorphisms allows elucidating the evolutionary history behind domestication, as well as discovering and isolating useful genes for molecular breeding. Deep transcriptome sequencing enables the exploration of sequence variations in transcribed sequences; such analysis is particularly useful for species with large and complex genomes, such as barley. In this study, we performed RNA sequencing of 20 barley accessions, comprising representatives of several biogeographic regions and a wild ancestor. We identified 38,729 to 79,949 SNPs in the 19 domesticated accessions and 55,403 SNPs in the wild barley and revealed their genome-wide distribution using a reference genome. Genome-scale comparisons among accessions showed a clear differentiation between oriental and occidental barley populations. The results based on population structure analyses provide genome-scale properties of sub-populations grouped to oriental, occidental and marginal groups in barley. Our findings suggest that the oriental population of domesticated barley has genomic variations distinct from those in occidental groups, which might have contributed to barley's domestication.
Volume 6
Pages 33199
Published 2016-9-12
DOI 10.1038/srep33199
PII srep33199
PMID 27616653
PMC PMC5018957
MeSH Base Sequence DNA, Plant / genetics Domestication Evolution, Molecular Genome, Plant Hordeum / genetics* Molecular Sequence Annotation Phylogeny Phylogeography Polymorphism, Single Nucleotide* Sequence Analysis, DNA Sequence Analysis, RNA Transcriptome
IF 3.998
Times Cited 6
Barley オオムギ系統