論文 - 詳細
| RRC ID | 47435 |
|---|---|
| 著者 | Senoo T, Kawano S, Ikeda S. |
| タイトル | DNA base excision repair and nucleotide excision repair synergistically contribute to survival of stationary-phase cells of the fission yeast Schizosaccharomyces pombe. |
| ジャーナル | Cell Biol Int |
| Abstract |
Defects of genome maintenance may causally contribute to aging. In general, base excision repair (BER) is involved in the repair of subtle base lesions and AP sites, and bulky helix-distorting lesions are restored by nucleotide excision repair (NER). Here, we measured the chronological lifespan (CLS) of BER- and NER-deficient mutants of the fission yeast Schizosaccharomyces pombe, and observed the aging process of cells. The CLS of the nth1 (gene for DNA glycosylase/AP lyase) mutant and the rad16 (a homolog of human XPF) mutant were slightly shorter than that of the wild-type (WT) strain. However, survival of the nth1Δ rad16Δ double mutant was significantly reduced after entry into the stationary phase. Deletion of rad16 in an AP endonuclease mutant apn2Δ also accelerated chronological aging. These results indicate that BER and NER synergistically contribute to genome maintenance in non-dividing cells. Reactive oxygen species (ROS) accumulated in cells during the stationary phase, and nth1Δ rad16Δ cells produced more ROS than WT cells. High mutation frequencies and nuclear DNA fragmentation were observed in nth1Δ rad16Δ stationary-phase cells concurrent with apoptotic-like cell death. Calorie restriction significantly reduced the level of ROS in the stationary phase and extended the CLS of nth1Δ rad16Δ cells. Therefore, ROS production critically affects the survival of the DNA repair mutant during chronological aging. |
| 巻・号 | 41(3) |
| ページ | 276-286 |
| 公開日 | 2017-3-1 |
| DOI | 10.1002/cbin.10722 |
| PMID | 28032397 |
| MeSH | Cell Survival / physiology Cells, Cultured DNA Damage / physiology* DNA Repair / physiology* Humans Reactive Oxygen Species / metabolism Schizosaccharomyces / cytology Schizosaccharomyces / physiology* Schizosaccharomyces pombe Proteins / physiology* |
| IF | 2.571 |
| 引用数 | 0 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY7507 FY6839 |