論文 - 詳細
| RRC ID | 47486 |
|---|---|
| 著者 | Katoh Y, Terada M, Nishijima Y, Takei R, Nozaki S, Hamada H, Nakayama K. |
| タイトル | Overall Architecture of the Intraflagellar Transport (IFT)-B Complex Containing Cluap1/IFT38 as an Essential Component of the IFT-B Peripheral Subcomplex. |
| ジャーナル | J Biol Chem |
| Abstract |
Intraflagellar transport (IFT) is essential for assembly and maintenance of cilia and flagella as well as ciliary motility and signaling. IFT is mediated by multisubunit complexes, including IFT-A, IFT-B, and the BBSome, in concert with kinesin and dynein motors. Under high salt conditions, purified IFT-B complex dissociates into a core subcomplex composed of at least nine subunits and at least five peripherally associated proteins. Using the visible immunoprecipitation assay, which we recently developed as a convenient protein-protein interaction assay, we determined the overall architecture of the IFT-B complex, which can be divided into core and peripheral subcomplexes composed of 10 and 6 subunits, respectively. In particular, we identified TTC26/IFT56 and Cluap1/IFT38, neither of which was included with certainty in previous models of the IFT-B complex, as integral components of the core and peripheral subcomplexes, respectively. Consistent with this, a ciliogenesis defect of Cluap1-deficient mouse embryonic fibroblasts was rescued by exogenous expression of wild-type Cluap1 but not by mutant Cluap1 lacking the binding ability to other IFT-B components. The detailed interaction map as well as comparison of subcellular localization of IFT-B components between wild-type and Cluap1-deficient cells provides insights into the functional relevance of the architecture of the IFT-B complex. |
| 巻・号 | 291(21) |
| ページ | 10962-75 |
| 公開日 | 2016-5-20 |
| DOI | 10.1074/jbc.M116.713883 |
| PII | S0021-9258(20)40842-7 |
| PMID | 26980730 |
| PMC | PMC4900248 |
| MeSH | Animals Biological Transport Cells, Cultured Flagella / metabolism* HEK293 Cells Humans Immunoprecipitation Intracellular Signaling Peptides and Proteins / chemistry Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism* Kinesins / metabolism Mice Mice, Knockout Multiprotein Complexes / chemistry Multiprotein Complexes / genetics Multiprotein Complexes / metabolism Protein Interaction Maps Protein Subunits |
| IF | 4.238 |
| 引用数 | 42 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pCAG-EGFP-C-IFT70B (RDB20234) pEGFP-C1-IFT54 (RDB20235) Genome Network Project Human cDNA Clone IRAK098M05 (HGX039493) IRAK068J01 (HGX027417). |