RRC ID 4755
Author Rosshart S, Hofmann M, Schweier O, Pfaff AK, Yoshimoto K, Takeuchi T, Molnar E, Schamel WW, Pircher H.
Title Interaction of KLRG1 with E-cadherin: new functional and structural insights.
Journal Eur J Immunol
Abstract The killer cell lectin-like receptor G1 (KLRG1) is an inhibitory receptor expressed by memory T cells and NK cells in man and mice. It is frequently used as a cell differentiation marker and members of the cadherin family are ligands for KLRG1. The present study provides new insights into the interaction of mouse KLRG1 with E-cadherin. Firstly, we demonstrate that co-engagement of KLRG1 and CD3/TCR in a spatially linked manner was required for inhibition arguing against the notion that KLRG1-ligation per se transmits inhibitory signals. Secondly, experiments with T cells carrying Y(7)F-mutant KLRG1 molecules with a replacement of the tyrosine residue to phenylalanine in the single ITIM indicated that the inhibitory activity of KLRG1 is counteracted to some degree by increased interaction of KLRG1(+) T cells with E-cadherin expressing target cells. Thirdly, we demonstrate that deletion of the first or the second external domain of E-cadherin abolished reactivity in KLRG1-reporter cell assays. Finally, we made the intriguing observation that KLRG1 formed multimeric protein complexes in T cells in addition to the previously described mono- and dimeric molecules.
Volume 38(12)
Pages 3354-64
Published 2008-12-1
DOI 10.1002/eji.200838690
PMID 19009530
MeSH Animals Antibodies / immunology Antigen-Presenting Cells / immunology CD3 Complex / immunology Cadherins / genetics Cadherins / metabolism* Cell Line Gene Deletion Genes, Reporter / genetics Mice Mutation / genetics Protein Binding Protein Multimerization Receptors, Antigen, T-Cell / immunology Receptors, Immunologic / genetics Receptors, Immunologic / metabolism*
IF 4.695
Times Cited 41
DNA material Mouse E-cadherin full length cDNA (RDB01184)