論文 - 詳細
| RRC ID | 4755 |
|---|---|
| 著者 | Rosshart S, Hofmann M, Schweier O, Pfaff AK, Yoshimoto K, Takeuchi T, Molnar E, Schamel WW, Pircher H. |
| タイトル | Interaction of KLRG1 with E-cadherin: new functional and structural insights. |
| ジャーナル | Eur J Immunol |
| Abstract |
The killer cell lectin-like receptor G1 (KLRG1) is an inhibitory receptor expressed by memory T cells and NK cells in man and mice. It is frequently used as a cell differentiation marker and members of the cadherin family are ligands for KLRG1. The present study provides new insights into the interaction of mouse KLRG1 with E-cadherin. Firstly, we demonstrate that co-engagement of KLRG1 and CD3/TCR in a spatially linked manner was required for inhibition arguing against the notion that KLRG1-ligation per se transmits inhibitory signals. Secondly, experiments with T cells carrying Y(7)F-mutant KLRG1 molecules with a replacement of the tyrosine residue to phenylalanine in the single ITIM indicated that the inhibitory activity of KLRG1 is counteracted to some degree by increased interaction of KLRG1(+) T cells with E-cadherin expressing target cells. Thirdly, we demonstrate that deletion of the first or the second external domain of E-cadherin abolished reactivity in KLRG1-reporter cell assays. Finally, we made the intriguing observation that KLRG1 formed multimeric protein complexes in T cells in addition to the previously described mono- and dimeric molecules. |
| 巻・号 | 38(12) |
| ページ | 3354-64 |
| 公開日 | 2008-12-1 |
| DOI | 10.1002/eji.200838690 |
| PMID | 19009530 |
| MeSH | Animals Antibodies / immunology Antigen-Presenting Cells / immunology CD3 Complex / immunology Cadherins / genetics Cadherins / metabolism* Cell Line Gene Deletion Genes, Reporter / genetics Lectins, C-Type Mice Mutation / genetics Protein Binding Protein Multimerization Receptors, Antigen, T-Cell / immunology Receptors, Immunologic / genetics Receptors, Immunologic / metabolism* |
| IF | 4.404 |
| 引用数 | 41 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 遺伝子材料 | Mouse E-cadherin full length cDNA (RDB01184) |