Reference - Detail
|Author||Klebanow LR, Peshel EC, Schuster AT, De K, Sarvepalli K, Lemieux ME, Lenoir JJ, Moore AW, McDonald JA, Longworth MS.|
|Title||Drosophila Condensin II subunit Chromosome-associated protein D3 regulates cell fate determination through non-cell-autonomous signaling.|
The pattern of the Drosophila melanogaster adult wing is heavily influenced by the expression of proteins that dictate cell fate decisions between intervein and vein during development. dSRF (Blistered) expression in specific regions of the larval wing disc promotes intervein cell fate, whereas EGFR activity promotes vein cell fate. Here, we report that the chromatin-organizing protein CAP-D3 acts to dampen dSRF levels at the anterior/posterior boundary in the larval wing disc, promoting differentiation of cells into the anterior crossvein. CAP-D3 represses KNOT expression in cells immediately adjacent to the anterior/posterior boundary, thus blocking KNOT-mediated repression of EGFR activity and preventing cell death. Maintenance of EGFR activity in these cells depresses dSRF levels in the neighboring anterior crossvein progenitor cells, allowing them to differentiate into vein cells. These findings uncover a novel transcriptional regulatory network influencing Drosophila wing vein development, and are the first to identify a Condensin II subunit as an important regulator of EGFR activity and cell fate determination in vivo.
|MeSH||Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism Animals Cell Cycle Proteins Cell Differentiation / genetics Cell Differentiation / physiology Chromatin Immunoprecipitation Chromosomes / genetics Chromosomes / metabolism* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Drosophila Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Fluorescent Antibody Technique Gene Expression Regulation, Developmental / genetics Gene Expression Regulation, Developmental / physiology In Situ Hybridization Multiprotein Complexes / genetics Multiprotein Complexes / metabolism Reverse Transcriptase Polymerase Chain Reaction Signal Transduction / genetics Signal Transduction / physiology|
|WOS Category||DEVELOPMENTAL BIOLOGY|