論文 - 詳細
| RRC ID | 47678 |
|---|---|
| 著者 | Ohkura M, Sasaki T, Kobayashi C, Ikegaya Y, Nakai J. |
| タイトル | An improved genetically encoded red fluorescent Ca2+ indicator for detecting optically evoked action potentials. |
| ジャーナル | PLoS One |
| Abstract |
Genetically encoded Ca(2+) indicators (GECIs) are powerful tools to image activities of defined cell populations. Here, we developed an improved red fluorescent GECI, termed R-CaMP1.07, by mutagenizing R-GECO1. In HeLa cell assays, R-CaMP1.07 exhibited a 1.5-2-fold greater fluorescence response compared to R-GECO1. In hippocampal pyramidal neurons, R-CaMP1.07 detected Ca(2+) transients triggered by single action potentials (APs) with a probability of 95% and a signal-to-noise ratio >7 at a frame rate of 50 Hz. The amplitudes of Ca(2+) transients linearly correlated with the number of APs. The expression of R-CaMP1.07 did not significantly alter the electrophysiological properties or synaptic activity patterns. The co-expression of R-CaMP1.07 and channelrhodpsin-2 (ChR2), a photosensitive cation channel, in pyramidal neurons demonstrated that R-CaMP1.07 was applicable for the monitoring of Ca(2+) transients in response to optically evoked APs, because the excitation light for R-CaMP1.07 hardly activated ChR2. These technical advancements provide a novel strategy for monitoring and manipulating neuronal activity with single cell resolution. |
| 巻・号 | 7(7) |
| ページ | e39933 |
| 公開日 | 2012-1-1 |
| DOI | 10.1371/journal.pone.0039933 |
| PII | PONE-D-12-12269 |
| PMID | 22808076 |
| PMC | PMC3393713 |
| MeSH | Action Potentials / physiology* Action Potentials / radiation effects Animals Animals, Newborn Calcium / analysis* Calcium / metabolism Channelrhodopsins Escherichia coli Fluorescent Dyes Gene Expression Genes, Reporter* HeLa Cells Humans Light Molecular Probes* Neurons / physiology* Neurons / radiation effects Pyramidal Cells / physiology* Pyramidal Cells / radiation effects Rats Rats, Wistar Recombinant Proteins / genetics Recombinant Proteins / metabolism Signal-To-Noise Ratio Single-Cell Analysis Transgenes |
| IF | 2.74 |
| 引用数 | 73 |
| WOS 分野 | NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 16 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | R-CaMP1.07 (RDB14608) |