RRC ID 47691
Author Ishida M, Marubashi S, Fukuda M.
Title M-INK, a novel tool for visualizing melanosomes and melanocores.
Journal J Biochem
Abstract Melanosome transfer from melanocytes to surrounding keratinocytes is one of the crucial but less well-characterized steps in the process of skin pigmentation. Although several markers have generally been used to detect melanosomes in melanocytes, no suitable markers for melanosomes that have been transferred into keratinocytes have ever been reported. The melanocore-interacting Kif1c-tail (M-INK) probe we developed and reported here specifically recognizes melanocores and thus makes visualizing melanosomes that have been incorporated into keratinocytes possible even in a fluorescent field. M-INK staining makes it possible to reconstruct 3D images of melanosome-containing keratinocytes, and thereby precisely localize melanosomes in keratinocytes.
Volume 161(4)
Pages 323-326
Published 2017-4-1
DOI 10.1093/jb/mvw100
PII mvw100
PMID 28096452
MeSH Animals Cell Line, Tumor Cells, Cultured Coculture Techniques Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Keratinocytes / metabolism* Kinesin / genetics Kinesin / metabolism* Melanocytes / metabolism* Melanosomes / metabolism* Mice Microscopy, Confocal Microscopy, Fluorescence Molecular Probes / genetics Molecular Probes / metabolism Mutation Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism
IF 2.476
Times Cited 3
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
DNA material pEF-Myc-Kif1c-tail-365-843 (RDB14632) pEF-Myc-Kif1c-tail-879-1100 (RDB14633) pEF-T7-GST-Kif1c-tail-879-1100/M-INK (RDB14634) pEF-Tyrosinase-FLAG (RDB14635).