論文 - 詳細
| RRC ID | 48002 |
|---|---|
| 著者 | Murakami S, Minami-Ohtsubo M, Nakato R, Shirahige K, Tabata T. |
| タイトル | Two Components of Aversive Memory in Drosophila, Anesthesia-Sensitive and Anesthesia-Resistant Memory, Require Distinct Domains Within the Rgk1 Small GTPase. |
| ジャーナル | J Neurosci |
| Abstract |
Multiple components have been identified that exhibit different stabilities for aversive olfactory memory in Drosophila These components have been defined by behavioral and genetic studies and genes specifically required for a specific component have also been identified. Intermediate-term memory generated after single cycle conditioning is divided into anesthesia-sensitive memory (ASM) and anesthesia-resistant memory (ARM), with the latter being more stable. We determined that the ASM and ARM pathways converged on the Rgk1 small GTPase and that the N-terminal domain-deleted Rgk1 was sufficient for ASM formation, whereas the full-length form was required for ARM formation. Rgk1 is specifically accumulated at the synaptic site of the Kenyon cells (KCs), the intrinsic neurons of the mushroom bodies, which play a pivotal role in olfactory memory formation. A higher than normal Rgk1 level enhanced memory retention, which is consistent with the result that Rgk1 suppressed Rac-dependent memory decay; these findings suggest that rgk1 bolsters ASM via the suppression of forgetting. We propose that Rgk1 plays a pivotal role in the regulation of memory stabilization by serving as a molecular node that resides at KC synapses, where the ASM and ARM pathway may interact.SIGNIFICANCE STATEMENT Memory consists of multiple components. Drosophila olfactory memory serves as a fundamental model with which to investigate the mechanisms that underlie memory formation and has provided genetic and molecular means to identify the components of memory, namely short-term, intermediate-term, and long-term memory, depending on how long the memory lasts. Intermediate memory is further divided into anesthesia-sensitive memory (ASM) and anesthesia-resistant memory (ARM), with the latter being more stable. We have identified a small GTPase in Drosophila, Rgk1, which plays a pivotal role in the regulation of olfactory memory stability. Rgk1 is required for both ASM and ARM. Moreover, N-terminal domain-deleted Rgk1 was sufficient for ASM formation, whereas the full-length form was required for ARM formation. |
| 巻・号 | 37(22) |
| ページ | 5496-5510 |
| 公開日 | 2017-5-31 |
| DOI | 10.1523/JNEUROSCI.3648-16.2017 |
| PII | JNEUROSCI.3648-16.2017 |
| PMID | 28416593 |
| PMC | PMC6596527 |
| MeSH | Anesthetics / administration & dosage* Animals Avoidance Learning / drug effects Avoidance Learning / physiology* Dose-Response Relationship, Drug Drosophila / drug effects Drosophila / physiology* Drosophila Proteins / chemistry* Drosophila Proteins / metabolism* Memory / drug effects Memory / physiology* Monomeric GTP-Binding Proteins / chemistry* Monomeric GTP-Binding Proteins / metabolism* Mushroom Bodies / drug effects Mushroom Bodies / physiology Protein Domains Sensory Receptor Cells / drug effects Sensory Receptor Cells / physiology Smell / drug effects Smell / physiology* Structure-Activity Relationship |
| IF | 5.674 |
| 引用数 | 3 |
| WOS 分野 | NEUROSCIENCES |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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