RRC ID 48057
著者 Tsai CR, Anderson AE, Burra S, Jo J, Galko MJ.
タイトル Yorkie regulates epidermal wound healing in Drosophila larvae independently of cell proliferation and apoptosis.
ジャーナル Dev. Biol.
Abstract Yorkie (Yki), the transcriptional co-activator of the Hippo signaling pathway, has well-characterized roles in balancing apoptosis and cell division during organ growth control. Yki is also required in diverse tissue regenerative contexts. In most cases this requirement reflects its well-characterized roles in balancing apoptosis and cell division. Whether Yki has repair functions outside of the control of cell proliferation, death, and growth is not clear. Here we show that Yki and Scalloped (Sd) are required for epidermal wound closure in the Drosophila larval epidermis. Using a GFP-tagged Yki transgene we show that Yki transiently translocates to some epidermal nuclei upon wounding. Genetic analysis strongly suggests that Yki interacts with the known wound healing pathway, Jun N-terminal kinase (JNK), but not with Platelet Derived Growth Factor/Vascular-Endothelial Growth Factor receptor (Pvr). Yki likely acts downstream of or parallel to JNK signaling and does not appear to regulate either proliferation or apoptosis in the larval epidermis during wound repair. Analysis of actin structures after wounding suggests that Yki and Sd promote wound closure through actin regulation. In sum, we found that Yki regulates an epithelial tissue repair process independently of its previously documented roles in balancing proliferation and apoptosis.
巻・号 427(1)
ページ 61-71
公開日 2017-5-18
DOI 10.1016/j.ydbio.2017.05.006
PII S0012-1606(17)30237-3
PMID 28514643
PMC PMC5713895
MeSH Animals Animals, Genetically Modified Apoptosis / genetics Apoptosis / physiology* Cell Proliferation / genetics Cell Proliferation / physiology* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila Proteins / physiology Drosophila melanogaster / genetics Drosophila melanogaster / metabolism Drosophila melanogaster / physiology* Epidermis / metabolism Epidermis / physiopathology* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Immunohistochemistry JNK Mitogen-Activated Protein Kinases / genetics JNK Mitogen-Activated Protein Kinases / metabolism Larva / genetics Larva / metabolism Larva / physiology Microscopy, Confocal Nuclear Proteins / genetics Nuclear Proteins / metabolism* RNA Interference Signal Transduction / genetics Signal Transduction / physiology Time Factors Trans-Activators / genetics Trans-Activators / metabolism* Transcription Factors / genetics Transcription Factors / metabolism Transcription Factors / physiology Wound Healing*
IF 2.944
引用数 0
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ショウジョウバエ 8544R-3 8544R-2 5680R-1 5680R-2 31196R-4 7788R-2 8624R-1 8091R-1 9553R-3 9553R-4