RRC ID 48093
Author Kawaguchi Y, Hoshimaru M, Nawa H, Nakanishi S.
Title Sequence analysis of cloned cDNA for rat substance P precursor: existence of a third substance P precursor.
Journal Biochem Biophys Res Commun
Abstract The sequence of the mRNA for the rat substance P precursor (preprotachykinin A) has been elucidated by molecular cloning and sequence analysis. The deduced amino acid sequence of rat preprotachykinin A indicates that it contains both substance P and substance K but differs in the sequence organization from either bovine alpha- or beta-preprotachykinin A reported previously. The existence of the bovine mRNA for the third preprotachykinin A has thus been examined and evidenced by the isolation of the corresponding cDNA clone. This mRNA, named gamma-preprotachykinin A mRNA, deletes the sequence precisely corresponding to the exon 4 sequence of the preprotachykinin A gene. Thus, alternative RNA splicing in the expression of the single preprotachykinin A gene results in the generation of three different forms of the preprotachykinin A mRNAs.
Volume 139(3)
Pages 1040-6
Published 1986-9-30
DOI 10.1016/s0006-291x(86)80282-0
PII S0006-291X(86)80282-0
PMID 2429656
MeSH Amino Acid Sequence Animals Base Sequence Cattle Cloning, Molecular DNA / analysis* DNA Restriction Enzymes / metabolism Neuropeptides / genetics* Protein Precursors / genetics* RNA, Messenger / analysis Rats Substance P / biosynthesis* Tachykinins*
IF 2.985
Times Cited 137
DNA material pRSP 6 gamma PPT (RDB13175)