RRC ID 48104
Author Tanaka K, Masu M, Nakanishi S.
Title Structure and functional expression of the cloned rat neurotensin receptor.
Journal Neuron
Abstract A functional cDNA clone for the rat neurotensin receptor was isolated by combining molecular cloning in an RNA expression vector with an electrophysiological assay in Xenopus oocytes. The neurotensin receptor consists of 424 amino acids with seven putative transmembrane domains and belongs to the family of G protein-coupled receptors. The cloned receptor expressed in mammalian cells or in Xenopus oocytes shows a selective and high-affinity binding to neurotensin peptides and undergoes potent desensitization by repeated application of neurotensin. The neurotensin receptor mRNA is expressed in both the brain and the peripheral tissues at different levels. This investigation discloses the molecular nature of the neurotensin receptor, which mediates the diverse neuronal and peripheral actions of neurotensin by effecting the G protein-associated second messenger system.
Volume 4(6)
Pages 847-54
Published 1990-6-1
DOI 10.1016/0896-6273(90)90137-5
PII 0896-6273(90)90137-5
PMID 1694443
MeSH Amino Acid Sequence Animals Base Sequence Binding, Competitive Cell Line Cloning, Molecular / methods Female GTP-Binding Proteins / genetics GTP-Binding Proteins / metabolism Gene Expression Kinetics Molecular Sequence Data Neurotensin / metabolism* Nucleic Acid Hybridization Oocytes / metabolism Organ Specificity Poly A / genetics RNA / genetics RNA, Messenger / genetics RNA, Messenger / metabolism Rats Receptors, Neurotensin Receptors, Neurotransmitter / genetics* Receptors, Neurotransmitter / metabolism Recombinant Proteins / metabolism Sequence Homology, Nucleic Acid Xenopus laevis
IF 14.415
Times Cited 485
DNA material prNTR 2 ( full8-6 ) (RDB13190)