RRC ID 4834
Author Ripoll JJ, Rodríguez-Cazorla E, González-Reig S, Andújar A, Alonso-Cantabrana H, Perez-Amador MA, Carbonell J, Martínez-Laborda A, Vera A.
Title Antagonistic interactions between Arabidopsis K-homology domain genes uncover PEPPER as a positive regulator of the central floral repressor FLOWERING LOCUS C.
Journal Dev. Biol.
Abstract Plant floral transition is a major developmental switch regulated by an integrated network of pathways. Arabidopsis FLOWERING LOCUS K (FLK), a protein with three KH RNA-binding domains, operates in the autonomous flowering-promotive pathway by decreasing the transcript levels of the key flowering repressor FLOWERING LOCUS C (FLC). Here we report that PEPPER (PEP), an FLK paralog previously shown to affect vegetative and pistil development, antagonizes FLK by positively regulating FLC. Lack of PEP function rescues the flk late-flowering phenotype with a concomitant decrease in FLC RNA levels. Loss of HUA2, another FLC activator encoding an RNA-binding protein, further rescues flk, being flk hua2 pep triple mutants virtually wild-type regarding flowering time. Consistently, PEP overexpression determines high levels of FLC transcripts and flowering delay. Genetic and molecular analyses indicate that FLK and PEP act independently of FCA, another important FLC repressor in the autonomous pathway. In addition, we present data suggesting that PEP may affect FLC expression at both transcriptional and post-transcriptional levels. Overall, our results uncover PEP as a new factor for FLC upregulation, underscoring the importance of RNA-binding activities during developmental timing of flowering.
Volume 333(2)
Pages 251-62
Published 2009-9-15
DOI 10.1016/j.ydbio.2009.06.035
PII S0012-1606(09)01024-0
PMID 19576878
MeSH Alleles Arabidopsis / genetics Arabidopsis / metabolism Arabidopsis / physiology* Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Arabidopsis Proteins / physiology Cell Nucleus / metabolism Gene Expression Regulation, Plant* Gibberellins / metabolism MADS Domain Proteins / genetics* MADS Domain Proteins / physiology Mutation* Phenotype Protein Structure, Tertiary RNA Processing, Post-Transcriptional RNA, Messenger / metabolism RNA-Binding Proteins / genetics* RNA-Binding Proteins / physiology Reverse Transcriptase Polymerase Chain Reaction Transcription, Genetic
IF 3.262
Times Cited 10
WOS Category DEVELOPMENTAL BIOLOGY
Resource
Arabidopsis / Cultured plant cells, genes pda04780