Reference - Detail
| RRC ID | 48764 |
|---|---|
| Author | Weston L, Greenwood J, Nurse P. |
| Title | Genome-wide screen for cell growth regulators in fission yeast. |
| Journal | J Cell Sci |
| Abstract |
Cellular growth control is important for all living organisms, but experimental investigation into this problem is difficult because of the complex range of growth regulatory mechanisms. Here, we have used the fission yeast Schizosaccharomyces pombe to identify potential master regulators of growth. At the restrictive temperature, the S. pombe pat1ts mei4Δ strain enters the meiotic developmental program, but arrests in meiotic G2 phase as mei4+ is essential for meiotic progression. These cells do not grow, even in an abundance of nutrients. To identify regulators of growth that can reverse this growth arrest, we introduced an ORFeome plasmid library into the pat1tsmei4Δ strain. Overexpression of eight genes promoted cell growth; two of these were core RNA polymerase subunits, and one was sck2+ , an S6 kinase thought to contribute to TORC1 signalling. Sck2 had the greatest effect on cell growth, and we also show that it significantly increases the cellular transcription rate. These findings indicate, for the first time, that global transcriptional control mediated through S6 kinase signalling is central to cellular growth control. |
| Volume | 130(12) |
| Pages | 2049-2055 |
| Published | 2017-6-15 |
| DOI | 10.1242/jcs.200865 |
| PII | jcs.200865 |
| PMID | 28476936 |
| PMC | PMC5482981 |
| MeSH | Cell Cycle DNA-Directed RNA Polymerases / metabolism Gene Expression Regulation, Fungal* Genome, Fungal* Open Reading Frames Phosphorylation Plasmids / metabolism Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism Ribosomal Protein S6 Kinases / genetics Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / metabolism Signal Transduction / genetics* |
| IF | 4.573 |
| Times Cited | 5 |
| WOS Category | CELL BIOLOGY |
| Resource | |
| DNA material | SpYFH01A01 (SPW084810) SpYFH26H06 (SPW090574) SpYFH46G08 (SPW098552) SpYFH41B01 (SPW096425) SpYFH35A12 (SPW094012) SpYFH50F03 (SPW100123) SpYFH07D07 (SPW082879) SpYFH45H04 (SPW098172) SpYFH29E06 (SPW091702) SpYFH13C06 (SPW085254) SpYFH19H03 (SPW087771) SpYFH24A04 (SPW089604) SpYFH24B10 (SPW089634) SpYFH37H07 (SPW094975) SpYFH22A09 (SPW088809) SpYFH48F07 (SPW099327) SpYFH32F09 (SPW092929) SpYFH21H09 (SPW088577) SpYFH33F01 (SPW093321) SpYFH12C09 (SPW084857) SpYFH22F07 (SPW088927) SpYFH14E12 (SPW085708) SpYFH36A12 (SPW094412) SpYFH12E06 (SPW084902) SpYFH32E04 (SPW092900) SpYFH12A10 (SPW080401). |