RRC ID 48769
Author Uhrig RG, Labandera AM, Tang LY, Sieben NA, Goudreault M, Yeung E, Gingras AC, Samuel MA, Moorhead GB.
Title Activation of Mitochondrial Protein Phosphatase SLP2 by MIA40 Regulates Seed Germination.
Journal Plant Physiol
Abstract Reversible protein phosphorylation catalyzed by protein kinases and phosphatases represents the most prolific and well-characterized posttranslational modification known. Here, we demonstrate that Arabidopsis (Arabidopsis thaliana) Shewanella-like protein phosphatase 2 (AtSLP2) is a bona fide Ser/Thr protein phosphatase that is targeted to the mitochondrial intermembrane space (IMS) where it interacts with the mitochondrial oxidoreductase import and assembly protein 40 (AtMIA40), forming a protein complex. Interaction with AtMIA40 is necessary for the phosphatase activity of AtSLP2 and is dependent on the formation of disulfide bridges on AtSLP2. Furthermore, by utilizing atslp2 null mutant, AtSLP2 complemented and AtSLP2 overexpressing plants, we identify a function for the AtSLP2-AtMIA40 complex in negatively regulating gibberellic acid-related processes during seed germination. Results presented here characterize a mitochondrial IMS-localized protein phosphatase identified in photosynthetic eukaryotes as well as a protein phosphatase target of the highly conserved eukaryotic MIA40 IMS oxidoreductase.
Volume 173(2)
Pages 956-969
Published 2017-2-1
DOI 10.1104/pp.16.01641
PII pp.16.01641
PMID 27923987
PMC PMC5291043
MeSH Abscisic Acid / pharmacology Amino Acid Sequence Arabidopsis / drug effects Arabidopsis / embryology* Arabidopsis / enzymology* Arabidopsis Proteins / chemistry Arabidopsis Proteins / metabolism* Biosynthetic Pathways / drug effects Disulfides / metabolism Enzyme Activation / drug effects Germination* / drug effects Gibberellins / biosynthesis Mitochondria / drug effects Mitochondria / enzymology* Mitochondrial Membranes / drug effects Mitochondrial Membranes / metabolism Mitochondrial Precursor Protein Import Complex Proteins Mitochondrial Proteins / chemistry Mitochondrial Proteins / metabolism* Models, Biological Oxidation-Reduction / drug effects Protein Binding / drug effects Protein Transport / drug effects Seeds / drug effects Seeds / embryology* Seeds / metabolism* Sequence Alignment Substrate Specificity / drug effects Triazoles / pharmacology
IF 6.902
Times Cited 13
Arabidopsis / Cultured plant cells, genes pst15755