論文 - 詳細
| RRC ID | 48814 |
|---|---|
| 著者 | Inagaki S, Tsutsui H, Suzuki K, Agetsuma M, Arai Y, Jinno Y, Bai G, Daniels MJ, Okamura Y, Matsuda T, Nagai T. |
| タイトル | Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging. |
| ジャーナル | Sci Rep |
| Abstract |
We report development of the first genetically encoded bioluminescent indicator for membrane voltage called LOTUS-V. Since it is bioluminescent, imaging LOTUS-V does not require external light illumination. This allows bidirectional optogenetic control of cellular activity triggered by Channelrhodopsin2 and Halorhodopsin during voltage imaging. The other advantage of LOTUS-V is the robustness of a signal-to-background ratio (SBR) wherever it expressed, even in the specimens where autofluorescence from environment severely interferes fluorescence imaging. Through imaging of moving cardiomyocyte aggregates, we demonstrated the advantages of LOTUS-V in long-term imaging are attributable to the absence of phototoxicity, and photobleaching in bioluminescent imaging, combined with the ratiometric aspect of LOTUS-V design. Collectively LOTUS-V extends the scope of excitable cell control and simultaneous voltage phenotyping, which should enable applications in bioscience, medicine and pharmacology previously not possible. |
| 巻・号 | 7 |
| ページ | 42398 |
| 公開日 | 2017-2-13 |
| DOI | 10.1038/srep42398 |
| PII | srep42398 |
| PMID | 28205521 |
| PMC | PMC5322354 |
| MeSH | Animals Cell Line Cells, Cultured Electrophysiological Phenomena Gene Expression* Genes, Reporter* Humans Induced Pluripotent Stem Cells / metabolism Kinetics Luminescent Measurements* / methods Luminescent Proteins / chemistry Luminescent Proteins / genetics* Luminescent Proteins / metabolism* Models, Molecular Molecular Imaging* / methods Optogenetics* / methods Protein Conformation |
| IF | 3.998 |
| 引用数 | 25 |
| WOS 分野 | NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 19 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 遺伝子材料 | CS-CDF-EG-PRE (RDB04380) pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393) LOTUS-V/pcDNA3 (RDB19881) |
| ヒト・動物細胞 | 293(RCB1637) 293T(RCB2202) |