Reference - Detail
| RRC ID | 49523 |
|---|---|
| Author | Itoh T, Fujita N, Kanno E, Yamamoto A, Yoshimori T, Fukuda M. |
| Title | Golgi-resident small GTPase Rab33B interacts with Atg16L and modulates autophagosome formation. |
| Journal | Mol Biol Cell |
| Abstract |
Macroautophagy is a mechanism of degradation of cytoplasmic components in all eukaryotic cells. In macroautophagy, cytoplasmic components are wrapped by double-membrane structures called autophagosomes, whose formation involves unique membrane dynamics, i.e., de novo formation of a double-membrane sac called the isolation membrane and its elongation. However, the precise regulatory mechanism of isolation membrane formation and elongation remains unknown. In this study, we showed that Golgi-resident small GTPase Rab33B (and Rab33A) specifically interacts with Atg16L, an essential factor in isolation membrane formation, in a guanosine triphosphate-dependent manner. Expression of a GTPase-deficient mutant Rab33B (Rab33B-Q92L) induced the lipidation of LC3, which is an essential process in autophagosome formation, even under nutrient-rich conditions, and attenuated macroautophagy, as judged by the degradation of p62/sequestosome 1. In addition, overexpression of the Rab33B binding domain of Atg16L suppressed autophagosome formation. Our findings suggest that Rab33 modulates autophagosome formation through interaction with Atg16L. |
| Volume | 19(7) |
| Pages | 2916-25 |
| Published | 2008-7-1 |
| DOI | 10.1091/mbc.e07-12-1231 |
| PII | E07-12-1231 |
| PMID | 18448665 |
| PMC | PMC2441679 |
| MeSH | Animals Autophagy Autophagy-Related Proteins COS Cells Carrier Proteins / metabolism* Cell Membrane / metabolism Chlorocebus aethiops GTP Phosphohydrolases / metabolism* Gene Expression Regulation, Enzymologic* Golgi Apparatus / metabolism* HeLa Cells Humans Mice NIH 3T3 Cells Phagosomes / metabolism* rab GTP-Binding Proteins / metabolism* |
| IF | 3.791 |
| Times Cited | 173 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | F1000Research |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pEF-T7-mouse Atg5 (RDB15374) pEF-T7-mouse Atg12 (RDB15375) pEF-T7-mouse Atg16L1gamma (RDB15376) pEGFP-C1-mouse Rab33A (RDB15378) pEGFP-C1-mouse Rab33B (RDB15379) pEGFP-C1-mouse Rab33B-Q92L (RDB15380) pEGFP-C1-mouse Rab33B-T47N (RDB15381). |