論文 - 詳細
| RRC ID | 49534 |
|---|---|
| 著者 | Fromental-Ramain C, Ramain P, Hamiche A. |
| タイトル | The Drosophila DAXX-Like Protein (DLP) Cooperates with ASF1 for H3.3 Deposition and Heterochromatin Formation. |
| ジャーナル | Mol Cell Biol |
| Abstract |
Histone variants are nonallelic isoforms of canonical histones, and they are deposited, in contrast to canonical histones, in a replication-independent (RI) manner. RI deposition of H3.3, a histone variant from the H3.3 family, is mediated in mammals by distinct pathways involving either the histone regulator A (HIRA) complex or the death-associated protein (DAXX)/α-thalassemia X-linked mental retardation protein (ATRX) complex. Here, we investigated the function of the Drosophila DAXX-like protein (DLP) by using both fly genetic approaches and protein biochemistry. DLP specifically interacts with H3.3 and shows a prominent localization on the base of the X chromosome, where it appears to act in concert with XNP, the Drosophila homolog of ATRX, in heterochromatin assembly and maintenance. The functional association between DLP and XNP is further supported by a series of experiments that illustrate genetic interactions and the DLP-XNP-dependent localization of specific chromosomal proteins. In addition, DLP both participates in the RI deposition of H3.3 and associates with anti-silencing factor 1 (ASF1). We suggest, in agreement with a recently proposed model, that DLP and ASF1 are part of a predeposition complex, which is recruited by XNP and is necessary to prevent DNA exposure in the nucleus. |
| 巻・号 | 37(12) |
| 公開日 | 2017-6-15 |
| DOI | 10.1128/MCB.00597-16 |
| PII | MCB.00597-16 |
| PMID | 28320872 |
| PMC | PMC5452728 |
| MeSH | Adaptor Proteins, Signal Transducing / metabolism* Animals Cell Cycle Proteins / metabolism* Chromosomes / metabolism DNA Helicases / metabolism DNA Replication Drosophila Proteins / metabolism* Drosophila melanogaster / embryology Drosophila melanogaster / metabolism* Embryo, Nonmammalian / metabolism Epistasis, Genetic Euchromatin / metabolism Female Heterochromatin / metabolism* Histones / metabolism* Male Models, Biological Mutation / genetics Nuclear Proteins / metabolism* Protein Binding |
| IF | 3.611 |
| 引用数 | 6 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 5825R-3 |