論文 - 詳細
| RRC ID | 49561 |
|---|---|
| 著者 | Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M. |
| タイトル | CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation. |
| ジャーナル | Nat Methods |
| Abstract |
Our improved CRISPR-Cas9-based photoactivatable transcription systems, CPTS2.0 and Split-CPTS2.0, enable high blue-light-inducible activation of endogenous target genes in various human cell lines. We achieved reversible activation of target genes with CPTS2.0 and induced neuronal differentiation in induced pluripotent stem cells (iPSCs) by upregulating NEUROD1 with Split-CPTS2.0. |
| 巻・号 | 14(10) |
| ページ | 963-966 |
| 公開日 | 2017-10-1 |
| DOI | 10.1038/nmeth.4430 |
| PII | nmeth.4430 |
| PMID | 28892089 |
| MeSH | CRISPR-Cas Systems* Cell Differentiation Cells, Cultured DNA, Complementary Fibroblasts Gene Expression Regulation / physiology Humans Light Luminescent Measurements Neurons / physiology* Optogenetics / methods* Transcription, Genetic |
| IF | 30.822 |
| 引用数 | 48 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 19 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pCAGGS (RDB08938). |
| ヒト・動物細胞 | HFSKF-II(RCB0698) 201B7(HPS0063) 454E2(HPS0077) |