RRC ID 49561
Author Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M.
Title CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation.
Journal Nat Methods
Abstract Our improved CRISPR-Cas9-based photoactivatable transcription systems, CPTS2.0 and Split-CPTS2.0, enable high blue-light-inducible activation of endogenous target genes in various human cell lines. We achieved reversible activation of target genes with CPTS2.0 and induced neuronal differentiation in induced pluripotent stem cells (iPSCs) by upregulating NEUROD1 with Split-CPTS2.0.
Volume 14(10)
Pages 963-966
Published 2017-10-1
DOI 10.1038/nmeth.4430
PII nmeth.4430
PMID 28892089
MeSH CRISPR-Cas Systems* Cell Differentiation Cells, Cultured DNA, Complementary Fibroblasts Gene Expression Regulation / physiology Humans Light Luminescent Measurements Neurons / physiology* Optogenetics / methods* Transcription, Genetic
IF 30.822
Times Cited 48
DNA material pCAGGS (RDB08938).
Human and Animal Cells HFSKF-II(RCB0698) 201B7(HPS0063) 454E2(HPS0077)