RRC ID 49667
Author Tanabe H, Seki M, Itoh M, Deepak A, Lal P, Horiuchi T, Suzuki Y, Kawakami K.
Title Fluorescence-Activated Cell Sorting and Gene Expression Profiling of GFP-Positive Cells from Transgenic Zebrafish Lines.
Journal Methods Mol Biol
Abstract Gene expression profiling is a useful approach for deeper understanding of the specificity of cells, tissues, and organs in the transcriptional level. Recent development of high-throughput next-generation sequence (NGS) allows the RNA-seq method for this profiling. This method provides precise information of transcripts about the quantitation and the structure such as the splicing variants. In this chapter, we describe a method for gene expression profiling of GFP-positive cells from transgenic zebrafish by RNA-seq. We labeled specific cells in the brain with GFP by crossing a Gal4 driver line with the UAS:GFP line, isolated those cells by fluorescence-activated cell sorting (FACS), and analyzed by RNA-seq.
Volume 1451
Pages 93-106
Published 2016-1-1
DOI 10.1007/978-1-4939-3771-4_7
PMID 27464803
MeSH Animals Animals, Genetically Modified / genetics Animals, Genetically Modified / metabolism* Enhancer Elements, Genetic / genetics* Flow Cytometry / methods* Gene Expression Profiling / methods* Gene Expression Regulation, Developmental / genetics Gene Expression Regulation, Developmental / physiology Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism* High-Throughput Nucleotide Sequencing Zebrafish / genetics Zebrafish / metabolism*
Times Cited 1