| RRC ID |
49667
|
| 著者 |
Tanabe H, Seki M, Itoh M, Deepak A, Lal P, Horiuchi T, Suzuki Y, Kawakami K.
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| タイトル |
Fluorescence-Activated Cell Sorting and Gene Expression Profiling of GFP-Positive Cells from Transgenic Zebrafish Lines.
|
| ジャーナル |
Methods Mol Biol
|
| Abstract |
Gene expression profiling is a useful approach for deeper understanding of the specificity of cells, tissues, and organs in the transcriptional level. Recent development of high-throughput next-generation sequence (NGS) allows the RNA-seq method for this profiling. This method provides precise information of transcripts about the quantitation and the structure such as the splicing variants. In this chapter, we describe a method for gene expression profiling of GFP-positive cells from transgenic zebrafish by RNA-seq. We labeled specific cells in the brain with GFP by crossing a Gal4 driver line with the UAS:GFP line, isolated those cells by fluorescence-activated cell sorting (FACS), and analyzed by RNA-seq.
|
| 巻・号 |
1451
|
| ページ |
93-106
|
| 公開日 |
2016-1-1
|
| DOI |
10.1007/978-1-4939-3771-4_7
|
| PMID |
27464803
|
| MeSH |
Animals
Animals, Genetically Modified / genetics
Animals, Genetically Modified / metabolism*
Enhancer Elements, Genetic / genetics*
Flow Cytometry / methods*
Gene Expression Profiling / methods*
Gene Expression Regulation, Developmental / genetics
Gene Expression Regulation, Developmental / physiology
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism*
High-Throughput Nucleotide Sequencing
Zebrafish / genetics
Zebrafish / metabolism*
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| 引用数 |
1
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| リソース情報 |
| ゼブラフィッシュ |
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