論文 - 詳細
| RRC ID | 50041 |
|---|---|
| 著者 | Shan L, Wu C, Chen D, Hou L, Li X, Wang L, Chu X, Hou Y, Wang Z. |
| タイトル | Regulators of alternative polyadenylation operate at the transition from mitosis to meiosis. |
| ジャーナル | J Genet Genomics |
| Abstract |
In the sexually reproductive organisms, gametes are produced by meiosis following a limited mitotic amplification. However, the intrinsic program switching cells from mitotic to meiotic cycle is unclear. Alternative polyadenylation (APA) is a highly conserved means of gene regulation and is achieved by the RNA 3'-processing machinery to generate diverse 3'UTR profiles. In Drosophila spermatogenesis, we observed distinct profiles of transcriptome-wide 3'UTR between mitotic and meiotic cells. In mutant germ cells stuck in mitosis, 3'UTRs of hundreds of genes were consistently shifted. Remarkably, altering the levels of multiple 3'-processing factors disrupted germline's progression to meiosis, indicative of APA's active role in this transition. An RNA-binding protein (RBP) Tut could directly bind 3'UTRs of 3'-processing factors whose expressions were repressed in the presence of Tut-containing complex. Further, we demonstrated that this RBP complex could execute the repression post-transcriptionally by recruiting CCR4/Twin of deadenylation complex. Thus, we propose that an RBP complex regulates the dynamic APA profile to promote the mitosis-to-meiosis transition. |
| 巻・号 | 44(2) |
| ページ | 95-106 |
| 公開日 | 2017-2-20 |
| DOI | 10.1016/j.jgg.2016.12.007 |
| PII | S1673-8527(17)30017-6 |
| PMID | 28190776 |
| MeSH | 3' Untranslated Regions / genetics Animals Drosophila melanogaster / cytology Drosophila melanogaster / genetics Drosophila melanogaster / metabolism Gene Expression Profiling Male Meiosis* Mitosis* Polyadenylation* RNA-Binding Proteins / metabolism |
| IF | 5.065 |
| 引用数 | 3 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 1972R-1 1972R-2 1109R-1 1109R-2 5222R-1 3642R-1 3642R-3 7697R-1 5970R-2 5970R-3 10228R-1 10228R-4 2163R-1 2163R-2 14213R-1 14213R-2 11486R-3 11486R-4 |