論文 - 詳細
RRC ID | 5014 |
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著者 | Ongol MP, Sawatari Y, Ebina Y, Sone T, Tanaka M, Tomita F, Yokota A, Asano K. |
タイトル | Yoghurt fermented by Lactobacillus delbrueckii subsp. bulgaricus H+ -ATPase-defective mutants exhibits enhanced viability of Bifidobacterium breve during storage. |
ジャーナル | Int J Food Microbiol |
Abstract |
Persistent acid production by Lactobacillus delbrueckii subsp. bulgaricus during refrigerated storage is a major cause of reduced viability of probiotic strains such as Bifidobacterium breve in yoghurt. It was established that H+ -ATPase-defective mutants of lactic acid bacteria have reduced growth and metabolism in low pH environments. Therefore, the aim of this study was to evaluate inhibition of post-acidification and maintenance of B. breve viability in yoghurt fermented by L. delbrueckii subsp. bulgaricus mutants with reduced membrane-bound H+ -ATPase activity during refrigerated storage. Spontaneous neomycin mutants of L. delbrueckii subsp. bulgaricus that had a significantly (P < or = 0.05) reduced H+ -ATPase activity were successfully isolated. Yoghurt fermented using L. delbrueckii subsp. bulgaricus SBT0164 No. 55-1 (mutant) starter culture had markedly reduced post-acidification and maintained viability (> or = 10(8) CFU/ml) of both Bifidobacteruim breve JCM 1192(T) and Bifidobacteruim breve JCM 7017 during storage at 10 degrees C for 21 days. These results clearly showed that yoghurt fermented by mutants of L. delbrueckii subsp. bulgaricus with reduced membrane-bound H+ -ATPase activity has reduced post-acidification that prolongs viability of B. breve in yoghurt during refrigerated storage. |
巻・号 | 116(3) |
ページ | 358-66 |
公開日 | 2007-5-30 |
DOI | 10.1016/j.ijfoodmicro.2007.02.019 |
PII | S0168-1605(07)00155-9 |
PMID | 17434219 |
MeSH | Bifidobacterium / growth & development* Colony Count, Microbial Fermentation Food Handling / methods* Food Preservation / methods* Humans Hydrogen-Ion Concentration Lactobacillus delbrueckii / genetics Lactobacillus delbrueckii / physiology* Mutation Proton-Translocating ATPases / deficiency* Proton-Translocating ATPases / genetics Temperature Time Factors Yogurt / microbiology* |
IF | 4.187 |
引用数 | 31 |
WOS 分野 | FOOD SCIENCE & TECHNOLOGY MICROBIOLOGY |
リソース情報 | |
一般微生物 | JCM1192 JCM7017 |