論文 - 詳細
| RRC ID | 50307 |
|---|---|
| 著者 | Myers TR, Amendola PG, Lussi YC, Salcini AE. |
| タイトル | JMJD-1.2 controls multiple histone post-translational modifications in germ cells and protects the genome from replication stress. |
| ジャーナル | Sci Rep |
| Abstract |
Post-translational modifications of histones, constitutive components of chromatin, regulate chromatin compaction and control all DNA-based cellular processes. C. elegans JMJD-1.2, a member of the KDM7 family, is a demethylase active towards several lysine residues on Histone 3 (H3), but its contribution in regulating histone methylation in germ cells has not been fully investigated. Here, we show that jmjd-1.2 is expressed abundantly in the germline where it controls the level of histone 3 lysine 9, lysine 23 and lysine 27 di-methylation (H3K9/K23/K27me2) both in mitotic and meiotic cells. Loss of jmjd-1.2 is not associated with major defects in the germ cells in animals grown under normal conditions or after DNA damage induced by UV or ionizing irradiation. However, jmjd-1.2 mutants are more sensitive to replication stress and the progeny of mutant animals exposed to hydroxyurea show increased embryonic lethality and mutational rate, compared to wild-type. Thus, our results suggest a role for jmjd-1.2 in the maintenance of genome integrity after replication stress and emphasize the relevance of the regulation of histone methylation in genomic stability. |
| 巻・号 | 8(1) |
| ページ | 3765 |
| 公開日 | 2018-2-28 |
| DOI | 10.1038/s41598-018-21914-9 |
| PII | 10.1038/s41598-018-21914-9 |
| PMID | 29491442 |
| PMC | PMC5830613 |
| MeSH | Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* DNA Damage / drug effects* DNA Replication / genetics* Genomic Instability Germ Cells / metabolism* Histones / metabolism* Jumonji Domain-Containing Histone Demethylases / genetics Jumonji Domain-Containing Histone Demethylases / metabolism* Mutation Protein Processing, Post-Translational* Protein Transport |
| IF | 3.998 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | jmjd-1.2(tm3713) |