RRC ID 50630
Author Gurudatta BV, Ramos E, Corces VG.
Title The BEAF insulator regulates genes involved in cell polarity and neoplastic growth.
Journal Dev Biol
Abstract Boundary Element Associated Factor-32 (BEAF-32) is an insulator protein predominantly found near gene promoters and thought to play a role in gene expression. We find that mutations in BEAF-32 are lethal, show loss of epithelial morphology in imaginal discs and cause neoplastic growth defects. To investigate the molecular mechanisms underlying this phenotype, we carried out a genome-wide analysis of BEAF-32 localization in wing imaginal disc cells. Mutation of BEAF-32 results in miss-regulation of 3850 genes by at least 1.5-fold, 794 of which are bound by this protein in wing imaginal cells. Up-regulated genes encode proteins involved in cell polarity, cell proliferation and cell differentiation. Among the down-regulated genes are those encoding components of the wingless pathway, which is required for cell differentiation. Miss-regulation of these genes explains the unregulated cell growth and neoplastic phenotypes observed in imaginal tissues of BEAF-32 mutants.
Volume 369(1)
Pages 124-32
Published 2012-9-1
DOI 10.1016/j.ydbio.2012.06.013
PII S0012-1606(12)00342-9
PMID 22743648
PMC PMC3572785
MeSH Animals Base Sequence Body Patterning / genetics Cell Differentiation / genetics Cell Polarity / genetics* DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / cytology* Drosophila melanogaster / genetics Epithelium / metabolism Epithelium / pathology Eye Proteins / genetics* Eye Proteins / metabolism Gene Expression Regulation, Neoplastic* Genes, Insect / genetics* Imaginal Discs / metabolism Insulator Elements / genetics* Molecular Sequence Data Mutation / genetics Neoplasms / genetics* Neoplasms / pathology Signal Transduction / genetics Transcription Initiation Site Transcription, Genetic Up-Regulation / genetics Wings, Animal / metabolism
IF 2.896
Times Cited 8