論文 - 詳細
RRC ID | 50630 |
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著者 | Gurudatta BV, Ramos E, Corces VG. |
タイトル | The BEAF insulator regulates genes involved in cell polarity and neoplastic growth. |
ジャーナル | Dev Biol |
Abstract |
Boundary Element Associated Factor-32 (BEAF-32) is an insulator protein predominantly found near gene promoters and thought to play a role in gene expression. We find that mutations in BEAF-32 are lethal, show loss of epithelial morphology in imaginal discs and cause neoplastic growth defects. To investigate the molecular mechanisms underlying this phenotype, we carried out a genome-wide analysis of BEAF-32 localization in wing imaginal disc cells. Mutation of BEAF-32 results in miss-regulation of 3850 genes by at least 1.5-fold, 794 of which are bound by this protein in wing imaginal cells. Up-regulated genes encode proteins involved in cell polarity, cell proliferation and cell differentiation. Among the down-regulated genes are those encoding components of the wingless pathway, which is required for cell differentiation. Miss-regulation of these genes explains the unregulated cell growth and neoplastic phenotypes observed in imaginal tissues of BEAF-32 mutants. |
巻・号 | 369(1) |
ページ | 124-32 |
公開日 | 2012-9-1 |
DOI | 10.1016/j.ydbio.2012.06.013 |
PII | S0012-1606(12)00342-9 |
PMID | 22743648 |
PMC | PMC3572785 |
MeSH | Animals Base Sequence Body Patterning / genetics Cell Differentiation / genetics Cell Polarity / genetics* DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / cytology* Drosophila melanogaster / genetics Epithelium / metabolism Epithelium / pathology Eye Proteins / genetics* Eye Proteins / metabolism Gene Expression Regulation, Neoplastic* Genes, Insect / genetics* Imaginal Discs / metabolism Insulator Elements / genetics* Molecular Sequence Data Mutation / genetics Neoplasms / genetics* Neoplasms / pathology Signal Transduction / genetics Transcription Initiation Site Transcription, Genetic Up-Regulation / genetics Wings, Animal / metabolism |
IF | 2.896 |
引用数 | 8 |
リソース情報 | |
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