論文 - 詳細
| RRC ID | 50772 |
|---|---|
| 著者 | Koelschbach JS, Mouttaki H, Pickl C, Heipieper HJ, Rachel R, Lawson PA, Meckenstock RU. |
| タイトル | Rectinema cohabitans gen. nov., sp. nov., a rod-shaped spirochaete isolated from an anaerobic naphthalene-degrading enrichment culture. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
The anaerobic, non-motile strain HMT was isolated from the naphthalene-degrading, sulfate-reducing enrichment culture N47. For 20 years, strain HMT has been a stable member of culture N47 although it is neither able to degrade naphthalene nor able to reduce sulfate in pure culture. The highest similarity of the 16S rRNA gene sequence of strain HMT (89 %) is with a cultivated member of the family Spirochaetaceae, Treponema caldariumstrain H1T (=DSM 7334T), an obligately anaerobic, thermophilic spirochaete isolated from cyanobacterial mat samples collected at a freshwater hot spring in Oregon, USA. In contrast to this strain and the majority of spirochaete species described, strain HMT showed a rod-shaped morphology. Growth occurred at temperatures between 12 and 50 °C (optimum 37 °C) but the isolate was not able to grow at 60 °C. The strain fermented various sugars including d-glucose, d-fructose, lactose and sucrose. Addition of 0.1 % (w/v) yeast extract or 0.1 % (w/v) tryptone to the culture medium was essential for growth and could not be replaced by either the vitamin solutions tested or by 0.1 % (w/v) peptone or 0.1 % (w/v) casamino acids. The DNA G+C content of the isolate was 51.5 mol%. The major fatty acids were C14 : 0, C18 : 1ω13c, C16 : 1ω9t, C16 : 1ω11c and C16 : 1ω9c. Based on the unique morphology and the phylogenetic distance from the closest cultivated relative, a novel genus and species, Rectinema cohabitans gen. nov., sp. nov., is proposed. The type strain is strain HMT (=DSM 100378T=JCM 30982T). |
| 巻・号 | 67(5) |
| ページ | 1288-1295 |
| 公開日 | 2017-5-1 |
| DOI | 10.1099/ijsem.0.001799 |
| PMID | 28100313 |
| MeSH | Amino Acids / chemistry Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Fatty Acids / chemistry Hot Springs / microbiology* Oregon Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Spirochaeta / classification* Spirochaeta / genetics Spirochaeta / isolation & purification Spirochaetales / genetics |
| IF | 2.415 |
| 引用数 | 6 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 30982 |