RRC ID 51282
Author Niklaus S, Cadetti L, Vom Berg-Maurer CM, Lehnherr A, Hotz AL, Forster IC, Gesemann M, Neuhauss SCF.
Title Shaping of Signal Transmission at the Photoreceptor Synapse by EAAT2 Glutamate Transporters.
Journal eNeuro
Abstract studies, we elucidate the role of excitatory amino acid transporter 2 (EAAT2) proteins in synaptic glutamate homeostasis at the zebrafish photoreceptor synapse. The main glutamate sink is provided by the glial EAAT2a, reflected by reduced electroretinographic responses in EAAT2a-depleted larvae. EAAT2b is located on the tips of cone pedicles and contributes little to glutamate reuptake. However, this transporter displays both a large chloride conductance and leak current, being important in stabilizing the cone resting potential. This work demonstrates not only how proteins originating from the same gene family can complement each other's expression profiles and biophysical properties, but also how presynaptic and glial transporters are coordinated to ensure efficient synaptic transmission at glutamatergic synapses of the central nervous system.
Volume 4(3)
Published 2017-5
DOI 10.1523/ENEURO.0339-16.2017
PII eN-NWR-0339-16
PMID 28612046
PMC PMC5467398
MeSH Animals Animals, Genetically Modified Excitatory Amino Acid Transporter 2 / genetics Excitatory Amino Acid Transporter 2 / metabolism* Eye / cytology Gene Expression Regulation / genetics Glutamic Acid / metabolism In Vitro Techniques Larva Morpholinos / genetics Morpholinos / metabolism Oocytes / physiology Patch-Clamp Techniques Photoreceptor Cells / classification* Photoreceptor Cells / metabolism* Presynaptic Terminals / metabolism* Retina / anatomy & histology Signal Transduction / drug effects Signal Transduction / genetics Synapses Synaptic Transmission / physiology Visual Pathways / physiology Xenopus Zebrafish
Resource
Zebrafish Tg(zfSWS1-5.5A:EGFP) Tg(zfSWS2-3.5A:EGFP)