| RRC ID |
51337
|
| 著者 |
Singh KD, Zheng X, Milstein S, Keller M, Roschitzki B, Grossmann J, Hengartner MO.
|
| タイトル |
Differential regulation of germ line apoptosis and germ cell differentiation by CPEB family members in C. elegans.
|
| ジャーナル |
PLoS One
|
| Abstract |
Cytoplasmic polyadenylation element binding (CPEB) proteins are evolutionary conserved RNA-binding proteins that control mRNA polyadenylation and translation. Orthologs in humans and other vertebrates are mainly involved in oogenesis. This is also the case for the C. elegans CPEB family member CPB-3, whereas two further CPEB proteins (CPB-1 and FOG-1) are involved in spermatogenesis. Here we describe the characterisation of a new missense allele of cpb-3 and show that loss of cpb-3 function leads to an increase in physiological germ cell death. To better understand the interaction and effect of C. elegans CPEB proteins on processes such as physiological apoptosis, germ cell differentiation, and regulation of gene expression, we characterised changes in the transcriptome and proteome of C. elegans CPEB mutants. Our results show that, despite their sequence similarities CPEB family members tend to have distinct overall effects on gene expression (both at the transcript and protein levels). This observation is consistent with the distinct phenotypes observed in the various CPEB family mutants.
|
| 巻・号 |
12(7)
|
| ページ |
e0182270
|
| 公開日 |
2017-7-31
|
| DOI |
10.1371/journal.pone.0182270
|
| PII |
PONE-D-17-07101
|
| PMID |
28759574
|
| PMC |
PMC5536308
|
| MeSH |
Animals
Apoptosis*
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Gene Expression Regulation, Developmental
Germ Cells / metabolism*
Male
Oogenesis
Phenotype
RNA-Binding Proteins / genetics
RNA-Binding Proteins / metabolism*
Spermatogenesis
Transcriptome
|
| IF |
2.74
|
| 引用数 |
3
|
| リソース情報 |
| 線虫 |
tm2821 |
