論文 - 詳細
| RRC ID | 51374 |
|---|---|
| 著者 | Yin J, Huang Y, Guo P, Hu S, Yoshina S, Xuan N, Gan Q, Mitani S, Yang C, Wang X. |
| タイトル | GOP-1 promotes apoptotic cell degradation by activating the small GTPase Rab2 in C. elegans. |
| ジャーナル | J Cell Biol |
| Abstract |
Apoptotic cells generated by programmed cell death are engulfed by phagocytes and enclosed within plasma membrane-derived phagosomes. Maturation of phagosomes involves a series of membrane-remodeling events that are governed by the sequential actions of Rab GTPases and lead to formation of phagolysosomes, where cell corpses are degraded. Here we identified gop-1 as a novel regulator of apoptotic cell clearance in Caenorhabditis elegans Loss of gop-1 affects phagosome maturation through the RAB-5-positive stage, causing defects in phagosome acidification and phagolysosome formation, phenotypes identical to and unaffected by loss of unc-108, the C. elegans Rab2 GOP-1 transiently associates with cell corpse-containing phagosomes, and loss of its function abrogates phagosomal association of UNC-108. GOP-1 interacts with GDP-bound and nucleotide-free UNC-108/Rab2, disrupts GDI-UNC-108 complexes, and promotes activation and membrane recruitment of UNC-108/Rab2 in vitro. Loss of gop-1 also abolishes association of UNC-108 with endosomes, causing defects in endosome and dense core vesicle maturation. Thus, GOP-1 is an activator of UNC-108/Rab2 in multiple processes. |
| 巻・号 | 216(6) |
| ページ | 1775-1794 |
| 公開日 | 2017-6-5 |
| DOI | 10.1083/jcb.201610001 |
| PII | jcb.201610001 |
| PMID | 28424218 |
| PMC | PMC5461019 |
| MeSH | Animals Animals, Genetically Modified Apoptosis Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Endocytosis Endosomes / enzymology Enzyme Activation Genotype Lectins, C-Type / genetics Lectins, C-Type / metabolism* Microscopy, Fluorescence Microscopy, Video Monosaccharide Transport Proteins / genetics Monosaccharide Transport Proteins / metabolism* Phagosomes / enzymology Phenotype Protein Binding Protein Transport Secretory Vesicles / enzymology Signal Transduction Time Factors Time-Lapse Imaging rab GTP-Binding Proteins / metabolism* rab2 GTP-Binding Protein / metabolism* rab5 GTP-Binding Proteins / genetics rab5 GTP-Binding Proteins / metabolism |
| IF | 8.811 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 16 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2095 tm5384 tm5654 tm5694 |