RRC ID 51382
Author Lee J, Yue Y, Park Y, Lee SH.
Title 3,3'-Diindolylmethane Suppresses Adipogenesis Using AMPKα-Dependent Mechanism in 3T3-L1 Adipocytes and Caenorhabditis elegans.
Journal J Med Food
Abstract 3,3'-diindolylmethane is a major in vivo metabolite of indole-3-carbinol, a bioactive compound found in cruciferous vegetables. Although 3,3'-diindolylmethane has been implicated to possess antitumorigenic and anti-inflammatory properties, the effect of 3,3'-diindolylmethane on adipogenesis has not been explored previously. Thus, the present study was conducted to determine if 3,3'-diindolylmethane affects adipogenesis using 3T3-L1 adipocytes and Caenorhabditis elegans. Treatment of 3,3'-diindolylmethane significantly reduced fat accumulation without affecting viability in 3T3-L1 adipocytes. 3,3'-diindolylmethane suppressed expression of peroxisome proliferator-activated receptor γ (PPARγ), CCAAT-enhancer-binding protein α (C/EBPα), fatty acid binding protein 4 (FABP4), and perilipin. In addition, 3,3'-diindolylmethane activated AMP-activated protein kinase α (AMPKα), which subsequently inactivated acetyl CoA carboxylase (ACC), resulting in reduced fat accumulation. These observations were further confirmed in C. elegans as treatment with 3,3'-diindolylmethane significantly reduced body fat accumulation, which was partly associated with aak-1, but not aak-2, orthologs of AMPKα catalytic subunits α1 and α2, respectively. The current results demonstrate that 3,3'-diindolylmethane, a biologically active metabolite of indole-3-carbinol, may prevent adipogenesis through the AMPKα-dependent pathway.
Volume 20(7)
Pages 646-652
Published 2017-7-1
DOI 10.1089/jmf.2016.0165
PMID 28459610
MeSH 3T3 Cells AMP-Activated Protein Kinases / genetics AMP-Activated Protein Kinases / metabolism* Adipocytes / cytology Adipocytes / drug effects* Adipocytes / enzymology Adipocytes / metabolism Adipogenesis / drug effects* Animals CCAAT-Enhancer-Binding Protein-alpha / genetics CCAAT-Enhancer-Binding Protein-alpha / metabolism Caenorhabditis elegans / drug effects Caenorhabditis elegans / enzymology Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Cell Survival / drug effects Fatty Acid-Binding Proteins / genetics Fatty Acid-Binding Proteins / metabolism Indoles / pharmacology* Mice PPAR gamma / genetics PPAR gamma / metabolism Triglycerides / metabolism
IF 2.04
Times Cited 4
C.elegans tm1944