論文 - 詳細
| RRC ID | 51410 |
|---|---|
| 著者 | Weaver BP, Weaver YM, Mitani S, Han M. |
| タイトル | Coupled Caspase and N-End Rule Ligase Activities Allow Recognition and Degradation of Pluripotency Factor LIN-28 during Non-Apoptotic Development. |
| ジャーナル | Dev Cell |
| Abstract |
Recent findings suggest that components of the classical cell death machinery also have important non-cell-death (non-apoptotic) functions in flies, nematodes, and mammals. However, the mechanisms for non-canonical caspase substrate recognition and proteolysis, and the direct roles for caspases in gene expression regulation, remain largely unclear. Here we report that CED-3 caspase and the Arg/N-end rule pathway cooperate to inactivate the LIN-28 pluripotency factor in seam cells, a stem-like cell type in Caenorhabditis elegans, thereby ensuring proper temporal cell fate patterning. Importantly, the caspase and the E3 ligase execute this function in a non-additive manner. We show that CED-3 caspase and the E3 ubiquitin ligase UBR-1 form a complex that couples their in vivo activities, allowing for recognition and rapid degradation of LIN-28 and thus facilitating a switch in developmental programs. The interdependence of these proteolytic activities provides a paradigm for non-apoptotic caspase-mediated protein inactivation. |
| 巻・号 | 41(6) |
| ページ | 665-673.e6 |
| 公開日 | 2017-6-19 |
| DOI | 10.1016/j.devcel.2017.05.013 |
| PII | S1534-5807(17)30395-7 |
| PMID | 28602583 |
| PMC | PMC5521180 |
| MeSH | Animals Apoptosis / physiology Caenorhabditis elegans / embryology Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Caspases / metabolism* Cell Differentiation / physiology* Embryonic Development / genetics* Gene Expression Regulation, Developmental / physiology* Proteolysis RNA-Binding Proteins / metabolism* Signal Transduction / physiology Ubiquitin-Protein Ligases / metabolism |
| IF | 10.092 |
| 引用数 | 19 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 18 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm5996 |