RRC ID 51472
著者 Brejc K, Bian Q, Uzawa S, Wheeler BS, Anderson EC, King DS, Kranzusch PJ, Preston CG, Meyer BJ.
タイトル Dynamic Control of X Chromosome Conformation and Repression by a Histone H4K20 Demethylase.
ジャーナル Cell
Abstract Chromatin modification and higher-order chromosome structure play key roles in gene regulation, but their functional interplay in controlling gene expression is elusive. We have discovered the machinery and mechanism underlying the dynamic enrichment of histone modification H4K20me1 on hermaphrodite X chromosomes during C. elegans dosage compensation and demonstrated H4K20me1's pivotal role in regulating higher-order chromosome structure and X-chromosome-wide gene expression. The structure and the activity of the dosage compensation complex (DCC) subunit DPY-21 define a Jumonji demethylase subfamily that converts H4K20me2 to H4K20me1 in worms and mammals. Selective inactivation of demethylase activity eliminates H4K20me1 enrichment in somatic cells, elevates X-linked gene expression, reduces X chromosome compaction, and disrupts X chromosome conformation by diminishing the formation of topologically associating domains (TADs). Unexpectedly, DPY-21 also associates with autosomes of germ cells in a DCC-independent manner to enrich H4K20me1 and trigger chromosome compaction. Our findings demonstrate the direct link between chromatin modification and higher-order chromosome structure in long-range regulation of gene expression.
巻・号 171(1)
ページ 85-102.e23
公開日 2017-9-21
DOI 10.1016/j.cell.2017.07.041
PII S0092-8674(17)30877-2
PMID 28867287
PMC PMC5678999
MeSH Amino Acid Sequence Animals Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / chemistry* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / chemistry* Carrier Proteins / genetics Carrier Proteins / metabolism* Dosage Compensation, Genetic Embryo, Nonmammalian / metabolism Gene Expression Regulation* Jumonji Domain-Containing Histone Demethylases / chemistry Jumonji Domain-Containing Histone Demethylases / metabolism Models, Molecular Mutation Piperidines / metabolism Sequence Alignment Thiophenes / metabolism X Chromosome / chemistry*
IF 38.637
引用数 20
リソース情報
線虫 tm1812