論文 - 詳細
| RRC ID | 51549 |
|---|---|
| 著者 | Ahier A, Dai CY, Tweedie A, Bezawork-Geleta A, Kirmes I, Zuryn S. |
| タイトル | Affinity purification of cell-specific mitochondria from whole animals resolves patterns of genetic mosaicism. |
| ジャーナル | Nat Cell Biol |
| Abstract |
Although mitochondria are ubiquitous organelles, they exhibit tissue-specific morphology, dynamics and function. Here, we describe a robust approach to isolate mitochondria from specific cells of diverse tissue systems in Caenorhabditis elegans. Cell-specific mitochondrial affinity purification (CS-MAP) yields intact and functional mitochondria with exceptional purity and sensitivity (>96% enrichment, >96% purity, and single-cell and single-animal resolution), enabling comparative analyses of protein and nucleic acid composition between organelles isolated from distinct cellular lineages. In animals harbouring a mixture of mutant and wild-type mitochondrial genomes, we use CS-MAP to reveal subtle mosaic patterns of cell-type-specific heteroplasmy across large populations of animals (>10,000 individuals). We demonstrate that the germline is more prone to propagating deleterious mitochondrial genomes than somatic lineages, which we propose is caused by enhanced mtDNA replication in this tissue. |
| 巻・号 | 20(3) |
| ページ | 352-360 |
| 公開日 | 2018-3-1 |
| DOI | 10.1038/s41556-017-0023-x |
| PII | 10.1038/s41556-017-0023-x |
| PMID | 29358705 |
| MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Cell Fractionation / methods* Chromatography, Affinity* DNA Replication DNA, Mitochondrial / biosynthesis DNA, Mitochondrial / genetics* Microscopy, Confocal Mitochondria / genetics* Mitochondria / metabolism Mosaicism* Mutation Organ Specificity |
| IF | 20.042 |
| 引用数 | 16 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 73 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1779 |