RRC ID 51791
Author Higuchi S, Yoshina S, Mitani S.
Title Inhibition of the integrin signal constitutes a mouse iPS cell niche.
Journal Dev Growth Differ
Abstract Stem cells are regulated by their surrounding microenvironments, called niche, such as cell-cell interaction and extracellular matrix. Classically, feeder cells as a niche have been used in the culture of iPS cells from both the mouse and the human. However, the regulation mechanism of stem cells by feeder cells as a niche still have been partially unclear. In this study, we used three murine iPS cell lines, iPS-MEF-Ng-20D-17, iPS-MEF-Ng-178B-5 and iPS-MEF-Fb/Ng-440A-3, which were generated by different reprogramming methods. In general, these cell lines commonly need the feeder cells as a niche to culture. Recently, the effect of substrate stiffness is known in stem cell study. First, we focused on the mechanical properties of feeder cells, and then we speculated that feeder-less culture might be made possible by using molecules in place of the mechanical properties of the niche. Finally, we found that the combination of disintegrin (echistatin) and 2i (GSK3 inhibitor and MEK inhibitor) is a sufficient condition for three murine iPS culture. This novel method of mimicking the murine iPS cell niche may be useful to understand signaling pathways to maintain the pluripotency of stem cells.
Volume 58(7)
Pages 586-99
Published 2016-9-1
DOI 10.1111/dgd.12302
PMID 27633818
MeSH Animals Cell Communication* / drug effects Cell Culture Techniques Cell Differentiation Cells, Cultured Cellular Reprogramming Glycogen Synthase Kinase 3 / antagonists & inhibitors Induced Pluripotent Stem Cells / drug effects Induced Pluripotent Stem Cells / physiology* Integrins / antagonists & inhibitors* Integrins / metabolism Intercellular Signaling Peptides and Proteins MAP Kinase Kinase Kinases / antagonists & inhibitors Mice Mouse Embryonic Stem Cells / cytology Mouse Embryonic Stem Cells / drug effects Mouse Embryonic Stem Cells / physiology Peptides / pharmacology Protein Kinase Inhibitors / pharmacology Signal Transduction / drug effects Stem Cell Niche / drug effects Stem Cell Niche / physiology*
IF 1.638
Times Cited 1
Human and Animal Cells iPS-MEF-Ng-20D-17(APS0001) iPS-MEF-Ng-178B-5(APS0002) iPS-MEF-Fb/Ng-440A-3(APS0003)