論文 - 詳細
| RRC ID | 51902 |
|---|---|
| 著者 | Nogi T, Mihara E, Yasui N, Takagi J. |
| タイトル | Immunoaffinity Purification of the Glycosylated Extracellular Fragment of Mouse Plexin A2 Produced in a Mammalian Expression System. |
| ジャーナル | Methods Mol Biol |
| Abstract |
Plexins are type I membrane proteins that function as receptors for semaphorins. All of the known plexins contain a large globular domain, termed the sema domain, in the N-terminal extracellular region, which interacts with semaphorins during signal transduction. Here, we describe procedures for protein production and purification that we utilized in the crystallographic study of the mouse Plexin A2 (mPlxnA2) extracellular fragment, including the sema domain. A mutant mammalian cell line, HEK293S GnTI-, was used as an expression host for the production of a crystallizable-quality mPlxnA2 fragment, which contains several N-glycosylation sites and disulfide bonds. |
| 巻・号 | 1493 |
| ページ | 57-72 |
| 公開日 | 2017-1-1 |
| DOI | 10.1007/978-1-4939-6448-2_4 |
| PMID | 27787842 |
| MeSH | Animals Chromatography, Affinity / methods* Crystallography, X-Ray Electrophoresis, Polyacrylamide Gel Glycosylation HEK293 Cells Humans Mice Mutation Nerve Tissue Proteins / chemistry Nerve Tissue Proteins / genetics Nerve Tissue Proteins / isolation & purification* Receptors, Cell Surface / chemistry Receptors, Cell Surface / genetics Receptors, Cell Surface / isolation & purification* Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / isolation & purification |
| 引用数 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | P20.1(RCB2815) |