論文 - 詳細
| RRC ID | 52175 |
|---|---|
| 著者 | Okuda T. |
| タイトル | PUGNAc treatment provokes globotetraosylceramide accumulation in human umbilical vein endothelial cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
PUGNAc is a well-investigated inhibitor for protein-O-GlcNAcase, whereas recent investigations showed that PUGNAc had a broad range as inhibitor for cellular β-hexosaminidases. Here we report that PUGNAc treatment provokes globotetraosylceramide (Gb4Cer) accumulation in human umbilical vein endothelial cells (HUVEC). HPLC analysis and a quantitative ELISA using newly developed anti-Gb4Cer monoclonal antibody revealed that PUGNAc treatment specifically increased the expression of Gb4Cer among glycosphingolipids expressed in HUVEC. Although the effect was weaker than PUGNAc, an O-GlcNAcase selective inhibitor (Thiamet-G) treatment also increased Gb4Cer levels in HUVEC. Furthermore, both of PUGNAc and Thiamet-G treatment up-regulated the expression levels of α-1,4-galactosyltransferase/Gb3Cer synthase gene which encodes a key enzyme in Gb4Cer synthesis. These results indicate that protein-O-GlcNAcylation can regulate the expression levels of cellular Gb4Cer. |
| 巻・号 | 487(1) |
| ページ | 76-82 |
| 公開日 | 2017-5-20 |
| DOI | 10.1016/j.bbrc.2017.04.019 |
| PII | S0006-291X(17)30675-7 |
| PMID | 28392398 |
| MeSH | Acetylglucosamine / administration & dosage Acetylglucosamine / analogs & derivatives* Animals Cells, Cultured Dose-Response Relationship, Drug Endothelial Cells / cytology Endothelial Cells / drug effects* Endothelial Cells / metabolism* Globosides / biosynthesis* Humans Mice Mice, Inbred C3H Oximes / administration & dosage* Phenylcarbamates / administration & dosage* Umbilical Veins / cytology* Umbilical Veins / drug effects Umbilical Veins / metabolism* Up-Regulation / drug effects Up-Regulation / genetics |
| IF | 2.985 |
| 引用数 | 8 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Sp2/0-Ag14(RCB0209) |