RRC ID 52259
Author Sekiguchi Y, Mano H, Nakatani S, Shimizu J, Kataoka A, Ogura K, Kimira Y, Ebata M, Wada M.
Title Mangiferin positively regulates osteoblast differentiation and suppresses osteoclast differentiation.
Journal Mol Med Rep
Abstract Mangiferin is a polyphenolic compound present in Salacia reticulata. It has been reported to reduce bone destruction and inhibit osteoclastic differentiation. This study aimed to determine whether mangiferin directly affects osteoblast and osteoclast proliferation and differentiation, and gene expression in MC3T3‑E1 osteoblastic cells and osteoclast‑like cells derived from primary mouse bone marrow macrophage cells. Mangiferin induced significantly greater WST‑1 activity, indicating increased cell proliferation. Mangiferin induced significantly increased alkaline phosphatase staining, indicating greater cell differentiation. Reverse transcription‑polymerase chain reaction (RT‑PCR) demonstrated that mangiferin significantly increased the mRNA level of runt‑related transcription factor 2 (RunX2), but did not affect RunX1 mRNA expression. Mangiferin significantly reduced the formation of tartrate‑resistant acid phosphatase‑positive multinuclear cells. RT‑PCR demonstrated that mangiferin significantly increased the mRNA level of estrogen receptor β (ERβ), but did not affect the expression of other osteoclast‑associated genes. Mangiferin may inhibit osteoclastic bone resorption by suppressing differentiation of osteoclasts and promoting expression of ERβ mRNA in mouse bone marrow macrophage cells. It also has potential to promote osteoblastic bone formation by promoting cell proliferation and inducing cell differentiation in preosteoblast MC3T3‑E1 cells via RunX2. Mangiferin may therefore be useful in improving bone disease outcomes.
Volume 16(2)
Pages 1328-1332
Published 2017-8
DOI 10.3892/mmr.2017.6752
PMID 28627701
PMC PMC5562065
MeSH Animals Biomarkers Bone Marrow Cells / cytology Bone Marrow Cells / drug effects Bone Marrow Cells / metabolism Cell Differentiation / drug effects* Cell Line Cell Lineage / drug effects Cell Lineage / genetics Cell Proliferation / drug effects Cells, Cultured Gene Expression Profiling Male Mice Osteoblasts / cytology* Osteoblasts / drug effects* Osteoblasts / metabolism Osteoclasts / cytology* Osteoclasts / drug effects* Osteoclasts / metabolism Xanthones / pharmacology*
IF 1.851
Times Cited 2
Human and Animal Cells MC3T3-E1(RCB1126)