| RRC ID |
52259
|
| 著者 |
Sekiguchi Y, Mano H, Nakatani S, Shimizu J, Kataoka A, Ogura K, Kimira Y, Ebata M, Wada M.
|
| タイトル |
Mangiferin positively regulates osteoblast differentiation and suppresses osteoclast differentiation.
|
| ジャーナル |
Mol Med Rep
|
| Abstract |
Mangiferin is a polyphenolic compound present in Salacia reticulata. It has been reported to reduce bone destruction and inhibit osteoclastic differentiation. This study aimed to determine whether mangiferin directly affects osteoblast and osteoclast proliferation and differentiation, and gene expression in MC3T3‑E1 osteoblastic cells and osteoclast‑like cells derived from primary mouse bone marrow macrophage cells. Mangiferin induced significantly greater WST‑1 activity, indicating increased cell proliferation. Mangiferin induced significantly increased alkaline phosphatase staining, indicating greater cell differentiation. Reverse transcription‑polymerase chain reaction (RT‑PCR) demonstrated that mangiferin significantly increased the mRNA level of runt‑related transcription factor 2 (RunX2), but did not affect RunX1 mRNA expression. Mangiferin significantly reduced the formation of tartrate‑resistant acid phosphatase‑positive multinuclear cells. RT‑PCR demonstrated that mangiferin significantly increased the mRNA level of estrogen receptor β (ERβ), but did not affect the expression of other osteoclast‑associated genes. Mangiferin may inhibit osteoclastic bone resorption by suppressing differentiation of osteoclasts and promoting expression of ERβ mRNA in mouse bone marrow macrophage cells. It also has potential to promote osteoblastic bone formation by promoting cell proliferation and inducing cell differentiation in preosteoblast MC3T3‑E1 cells via RunX2. Mangiferin may therefore be useful in improving bone disease outcomes.
|
| 巻・号 |
16(2)
|
| ページ |
1328-1332
|
| 公開日 |
2017-8-1
|
| DOI |
10.3892/mmr.2017.6752
|
| PMID |
28627701
|
| PMC |
PMC5562065
|
| MeSH |
Animals
Biomarkers
Bone Marrow Cells / cytology
Bone Marrow Cells / drug effects
Bone Marrow Cells / metabolism
Cell Differentiation / drug effects*
Cell Line
Cell Lineage / drug effects
Cell Lineage / genetics
Cell Proliferation / drug effects
Cells, Cultured
Gene Expression Profiling
Male
Mice
Osteoblasts / cytology*
Osteoblasts / drug effects*
Osteoblasts / metabolism
Osteoclasts / cytology*
Osteoclasts / drug effects*
Osteoclasts / metabolism
Xanthones / pharmacology*
|
| IF |
2.1
|
| 引用数 |
2
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
