| RRC ID |
52710
|
| 著者 |
Charney RM, Forouzmand E, Cho JS, Cheung J, Paraiso KD, Yasuoka Y, Takahashi S, Taira M, Blitz IL, Xie X, Cho KW.
|
| タイトル |
Foxh1 Occupies cis-Regulatory Modules Prior to Dynamic Transcription Factor Interactions Controlling the Mesendoderm Gene Program.
|
| ジャーナル |
Dev Cell
|
| Abstract |
The interplay between transcription factors and chromatin dictates gene regulatory network activity. Germ layer specification is tightly coupled with zygotic gene activation and, in most metazoans, is dependent upon maternal factors. We explore the dynamic genome-wide interactions of Foxh1, a maternal transcription factor that mediates Nodal/TGF-β signaling, with cis-regulatory modules (CRMs) during mesendodermal specification. Foxh1 marks CRMs during cleavage stages and recruits the co-repressor Tle/Groucho in the early blastula. We highlight a population of CRMs that are continuously occupied by Foxh1 and show that they are marked by H3K4me1, Ep300, and Fox/Sox/Smad motifs, suggesting interplay between these factors in gene regulation. We also propose a molecular "hand-off" between maternal Foxh1 and zygotic Foxa at these CRMs to maintain enhancer activation. Our findings suggest that Foxh1 functions at the top of a hierarchy of interactions by marking developmental genes for activation, beginning with the onset of zygotic gene expression.
|
| 巻・号 |
40(6)
|
| ページ |
595-607.e4
|
| 公開日 |
2017-3-27
|
| DOI |
10.1016/j.devcel.2017.02.017
|
| PII |
S1534-5807(17)30086-2
|
| PMID |
28325473
|
| PMC |
PMC5434453
|
| MeSH |
Animals
Blastula / metabolism
Cleavage Stage, Ovum / metabolism
Co-Repressor Proteins / metabolism
Embryo, Nonmammalian / metabolism
Endoderm / embryology
Endoderm / metabolism*
Enhancer Elements, Genetic / genetics
Forkhead Transcription Factors / genetics
Forkhead Transcription Factors / metabolism*
Gene Expression Regulation, Developmental*
Genome
Histones / metabolism
Lysine / metabolism
Mesoderm / embryology
Mesoderm / metabolism*
Methylation
Nodal Protein / metabolism
Protein Binding / genetics
RNA Polymerase II / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism
Regulatory Sequences, Nucleic Acid / genetics
Sequence Analysis, RNA
Signal Transduction / genetics
Transcription Factors / metabolism*
Transcription, Genetic
Xenopus / embryology*
Xenopus / genetics*
Xenopus / metabolism
Xenopus Proteins / genetics
Xenopus Proteins / metabolism*
|
| IF |
10.092
|
| 引用数 |
20
|
| リソース情報 |
| ツメガエル・イモリ |
Xenopus tropicalis |
