RRC ID 5307
Author Namwong S, Tanasupawat S, Visessanguan W, Kudo T, Itoh T.
Title Halococcus thailandensis sp. nov., from fish sauce in Thailand.
Journal Int. J. Syst. Evol. Microbiol.
Abstract Fifteen strains of red-pigmented, strictly aerobic, coccoid, extremely halophilic archaea were isolated from fish sauce (nam-pla) produced in Thailand. They grew optimally at 37 degrees C, pH 6-8 and in the presence of 20-30 % (w/v) NaCl. The DNA G+C contents of the isolates were 60.0-61.8 mol%. They had MK-8(H2) as a major menaquinone component and C(20)C(20) and C(20)C(25) derivatives of phosphatidylglycerol, phosphatidylglycerol methylphosphate and a sulfated glycolipid, S-DGA-1, as major polar lipid components. 16S rRNA gene sequence comparisons revealed that a representative strain, HDB5-2(T), was affiliated with Halococcus dombrowskii JCM 12289(T), Halococcus qingdaonensis JCM 13587(T) and Halococcus morrhuae JCM 8876(T) (levels of similarity of 98.2-98.7 %). Based on data from DNA-DNA hybridization experiments, the 15 strains represented a single species, showing hybridization values of >78.9 % to representative strain HDB5-2(T), but were unrelated to either Halococcus dombrowskii JCM 12289(T) or Halococcus morrhuae JCM 8876(T), with levels of relatedness of <50 %. Moreover, a comparison of phenotypic properties discriminated these new isolates from recognized species of the genus Halococcus. The 15 strains are thus considered to represent a novel species of the genus Halococcus, for which the name Halococcus thailandensis sp. nov. is proposed. The type strain is HDB5-2(T) (=BCC 20213(T) =JCM 13552(T) =PCU 278(T)).
Volume 57(Pt 10)
Pages 2199-2203
Published 2007-10
DOI 10.1099/ijs.0.65218-0
PMID 17911282
MeSH Aerobiosis Base Composition DNA, Archaeal / chemistry DNA, Archaeal / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fish Products / microbiology* Food Microbiology Genes, rRNA Glycolipids / analysis Halococcus / chemistry Halococcus / classification* Halococcus / genetics Halococcus / isolation & purification* Hydrogen-Ion Concentration Molecular Sequence Data Nucleic Acid Hybridization Phospholipids / analysis Phylogeny RNA, Archaeal / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid Sodium Chloride / metabolism Temperature Thailand Vitamin K 2 / analysis
IF 2.166
Times Cited 23
WOS Category MICROBIOLOGY
Resource
General Microbes JCM12289 JCM8876 JCM8878 JCM13552