論文 - 詳細
| RRC ID | 53110 |
|---|---|
| 著者 | Htet AN, Noguchi M, Ninomiya K, Tsuge Y, Kuroda K, Kajita S, Masai E, Katayama Y, Shikinaka K, Otsuka Y, Nakamura M, Honda R, Takahashi K. |
| タイトル | Application of microalgae hydrolysate as a fermentation medium for microbial production of 2-pyrone 4,6-dicarboxylic acid. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
Actual biomass of microalgae was tested as a fermentation substrate for microbial production of 2-pyrone 4,6-dicarboxylic acid (PDC). Acid-hydrolyzed green microalgae Chlorella emersonii (algae hydrolysate) was diluted to adjust the glucose concentration to 2 g/L and supplemented with the nutrients of Luria-Bertani (LB) medium (tryptone 10 g/L and yeast extract 5 g/L). When the algae hydrolysate was used as a fermentation source for recombinant Escherichia coli producing PDC, 0.43 g/L PDC was produced with a yield of 20.1% (mol PDC/mol glucose), whereas 0.19 g/L PDC was produced with a yield of 8.6% when LB medium supplemented with glucose was used. To evaluate the potential of algae hydrolysate alone as a fermentation medium for E. coli growth and PDC production, the nutrients of LB medium were reduced from the algae hydrolysate medium. Interestingly, 0.17 g/L PDC was produced even without additional nutrient, which was comparable to the case using pure glucose medium with nutrients of LB medium. When using a high concentration of hydrolysate without additional nutrients, 1.22 g/L PDC was produced after a 24-h cultivation with the yield of 16.1%. Overall, C. emersonii has high potential as cost-effective fermentation substrate for the microbial production of PDC. |
| 巻・号 | 125(6) |
| ページ | 717-722 |
| 公開日 | 2018-6-1 |
| DOI | 10.1016/j.jbiosc.2017.12.026 |
| PII | S1389-1723(17)31105-2 |
| PMID | 29395960 |
| MeSH | Biomass Carboxylic Ester Hydrolases / metabolism Chlorella / enzymology Chlorella / growth & development Chlorella / metabolism* Escherichia coli / genetics Escherichia coli / metabolism* Fermentation* Glucose / metabolism Hydrolysis Microalgae / enzymology Microalgae / growth & development Microalgae / metabolism* Organisms, Genetically Modified Pyrones / metabolism* |
| IF | 2.366 |
| 引用数 | 6 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-2151 |