論文 - 詳細
| RRC ID | 5315 |
|---|---|
| 著者 | Yabe D, Fukuda H, Aoki M, Yamada S, Takebayashi S, Shinkura R, Yamamoto N, Honjo T. |
| タイトル | Generation of a conditional knockout allele for mammalian Spen protein Mint/SHARP. |
| ジャーナル | Genesis |
| Abstract |
The Spen protein family is found in worms, flies, and mammals, and is implicated in diverse biological processes from embryogenesis to aging. Spen proteins have three N-terminal RNA recognition motifs and a C-terminal SPOC domain. The mammalian Spen proteins Mint and its human ortholog SHARP interact with the Notch-signaling mediator RBP-J as well as Msx2 and several unliganded nuclear hormone receptors, and impart transcription-repressing activity to these molecules by recruiting corepressors through the SPOC domain. Despite these in vitro findings, Mint/SHARP's physiological role is largely unknown, because Mint germline knockouts are embryonic lethal. To analyze Mint/SHARP function in postnatal mice, we created Mint-floxed mice that allow the Cre/loxP-mediated conditional knockout of Mint. We analyzed Mint and RBP-J epistasis during Notch-dependent splenic B-lymphocyte development, and found that Mint suppresses Notch signaling through RBP-J. In addition, Mint deficiency caused severe hypoplasia in postnatal brain, suggesting it may regulate neuronal cell survival. |
| 巻・号 | 45(5) |
| ページ | 300-6 |
| 公開日 | 2007-5-1 |
| DOI | 10.1002/dvg.20296 |
| PMID | 17457934 |
| MeSH | Animals B-Lymphocytes / cytology B-Lymphocytes / metabolism Brain / metabolism Brain / pathology DNA-Binding Proteins / metabolism Gene Targeting / methods Homeodomain Proteins / genetics Humans In Situ Hybridization Mice Mice, Inbred C57BL Mice, Inbred CBA Mice, Knockout Mice, Transgenic Nuclear Proteins / deficiency* Nuclear Proteins / genetics* Nuclear Proteins / metabolism RNA-Binding Proteins Receptors, Notch / metabolism Signal Transduction |
| IF | 1.76 |
| 引用数 | 21 |
| WOS 分野 | GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 実験動物マウス | RBRC00254 |