RRC ID 53217
Author Kondo A, Fujiwara T, Okitsu Y, Fukuhara N, Onishi Y, Nakamura Y, Sawada K, Harigae H.
Title Identification of a novel putative mitochondrial protein FAM210B associated with erythroid differentiation.
Journal Int J Hematol
Abstract The transcription factor GATA-1 plays an essential role in erythroid differentiation. To identify novel GATA-1 target genes, we analyzed a merged ChIP-seq and expression profiling dataset. We identified FAM210B as a putative novel GATA-1 target gene. Study results demonstrated that GATA-1 directly regulates FAM210B expression, presumably by binding to an intronic enhancer region. Both human and murine FAM210B are abundantly expressed in the later stages of erythroblast development. Moreover, the deduced amino acid sequence predicted that FAM210B is a membrane protein, and Western blot analysis demonstrated its mitochondrial localization. Loss-of-function analysis in erythroid cells suggested that FAM210B may be involved in erythroid differentiation. The identification and characterization of FAM210B provides new insights in the study of erythropoiesis and hereditary anemias.
Volume 103(4)
Pages 387-95
Published 2016-4-1
DOI 10.1007/s12185-016-1968-4
PII 10.1007/s12185-016-1968-4
PMID 26968549
MeSH Cell Line Erythroblasts / cytology* Erythroblasts / metabolism Erythroid Cells / cytology* Erythroid Cells / metabolism Erythropoiesis* GATA1 Transcription Factor / genetics GATA1 Transcription Factor / metabolism* Gene Expression Regulation, Developmental HEK293 Cells Humans Introns Membrane Proteins / genetics Membrane Proteins / metabolism* Mitochondrial Proteins / genetics Mitochondrial Proteins / metabolism* RNA Interference RNA, Small Interfering / genetics
IF 2.245
Times Cited 4
Cord blood stem cells for research