Reference - Detail
| RRC ID | 53244 |
|---|---|
| Author | Chen Y, Bharill S, Altun Z, O'Hagan R, Coblitz B, Isacoff EY, Chalfie M. |
| Title | Caenorhabditis elegans paraoxonase-like proteins control the functional expression of DEG/ENaC mechanosensory proteins. |
| Journal | Mol Biol Cell |
| Abstract |
Caenorhabditis eleganssenses gentle touch via a mechanotransduction channel formed from the DEG/ENaC proteins MEC-4 and MEC-10. An additional protein, the paraoxonase-like protein MEC-6, is essential for transduction, and previous work suggested that MEC-6 was part of the transduction complex. We found that MEC-6 and a similar protein, POML-1, reside primarily in the endoplasmic reticulum and do not colocalize with MEC-4 on the plasma membrane in vivo. As with MEC-6, POML-1 is needed for touch sensitivity, the neurodegeneration caused by themec-4(d)mutation, and the expression and distribution of MEC-4 in vivo. Both proteins are likely needed for the proper folding or assembly of MEC-4 channels in vivo as measured by FRET. MEC-6 detectably increases the rate of MEC-4 accumulation on theXenopusoocyte plasma membrane. These results suggest that MEC-6 and POML-1 interact with MEC-4 to facilitate expression and localization of MEC-4 on the cell surface. Thus MEC-6 and POML-1 act more like chaperones for MEC-4 than channel components. |
| Volume | 27(8) |
| Pages | 1272-85 |
| Published | 2016-4-15 |
| DOI | 10.1091/mbc.E15-08-0561 |
| PII | mbc.E15-08-0561 |
| PMID | 26941331 |
| PMC | PMC4831881 |
| MeSH | Animals Animals, Genetically Modified Aryldialkylphosphatase / genetics Aryldialkylphosphatase / metabolism* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Membrane / metabolism Endoplasmic Reticulum / metabolism Female Fluorescence Resonance Energy Transfer Membrane Proteins / genetics Membrane Proteins / metabolism* Mutation Neurons / metabolism Neurons / pathology Oocytes / metabolism Xenopus laevis |
| IF | 3.791 |
| Times Cited | 12 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm4234 |