RRC ID 53250
Author Gerson-Gurwitz A, Wang S, Sathe S, Green R, Yeo GW, Oegema K, Desai A.
Title A Small RNA-Catalytic Argonaute Pathway Tunes Germline Transcript Levels to Ensure Embryonic Divisions.
Journal Cell
Abstract Multiple division cycles without growth are a characteristic feature of early embryogenesis. The female germline loads proteins and RNAs into oocytes to support these divisions, which lack many quality control mechanisms operating in somatic cells undergoing growth. Here, we describe a small RNA-Argonaute pathway that ensures early embryonic divisions in C. elegans by employing catalytic slicing activity to broadly tune, instead of silence, germline gene expression. Misregulation of one target, a kinesin-13 microtubule depolymerase, underlies a major phenotype associated with pathway loss. Tuning of target transcript levels is guided by the density of homologous small RNAs, whose generation must ultimately be related to target sequence. Thus, the tuning action of a small RNA-catalytic Argonaute pathway generates oocytes capable of supporting embryogenesis. We speculate that the specialized nature of germline chromatin led to the emergence of small RNA-catalytic Argonaute pathways in the female germline as a post-transcriptional control layer to optimize oocyte composition.
Volume 165(2)
Pages 396-409
Published 2016-4-7
DOI 10.1016/j.cell.2016.02.040
PII S0092-8674(16)30190-8
PMID 27020753
PMC PMC4826293
MeSH Animals Argonaute Proteins / metabolism Base Sequence Caenorhabditis elegans / cytology Caenorhabditis elegans / embryology* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism Cell Division Embryo, Nonmammalian / cytology Embryo, Nonmammalian / metabolism* Embryonic Development Female Kinesin / metabolism Metabolic Networks and Pathways* Microtubules / metabolism Molecular Sequence Data Oocytes / metabolism* RNA Processing, Post-Transcriptional
IF 36.216
Times Cited 29
C.elegans tm892