RRC ID 53263
Author Truttmann MC, Cruz VE, Guo X, Engert C, Schwartz TU, Ploegh HL.
Title The Caenorhabditis elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones.
Journal PLoS Genet
Abstract Protein AMPylation by Fic domain-containing proteins (Fic proteins) is an ancient and conserved post-translational modification of mostly unexplored significance. Here we characterize the Caenorhabditis elegans Fic protein FIC-1 in vitro and in vivo. FIC-1 is an AMPylase that localizes to the nuclear surface and modifies core histones H2 and H3 as well as heat shock protein 70 family members and translation elongation factors. The three-dimensional structure of FIC-1 is similar to that of its human ortholog, HYPE, with 38% sequence identity. We identify a link between FIC-1-mediated AMPylation and susceptibility to the pathogen Pseudomonas aeruginosa, establishing a connection between AMPylation and innate immunity in C. elegans.
Volume 12(5)
Pages e1006023
Published 2016-5-1
DOI 10.1371/journal.pgen.1006023
PMID 27138431
PMC PMC4854385
MeSH Animals Caenorhabditis elegans / chemistry Caenorhabditis elegans / genetics* Caenorhabditis elegans / microbiology Caenorhabditis elegans Proteins / genetics* Carrier Proteins / chemistry Carrier Proteins / genetics Crystallography, X-Ray DNA-Binding Proteins / chemistry DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism HSP70 Heat-Shock Proteins / chemistry HSP70 Heat-Shock Proteins / genetics HSP70 Heat-Shock Proteins / metabolism Heat-Shock Response / genetics Histones / genetics Histones / metabolism Humans Immunity, Innate / genetics Membrane Proteins / chemistry Membrane Proteins / genetics Nucleotidyltransferases / genetics* Peptide Chain Elongation, Translational* Protein Conformation Pseudomonas aeruginosa / genetics Pseudomonas aeruginosa / pathogenicity Recombinant Proteins / chemistry Recombinant Proteins / genetics* Recombinant Proteins / metabolism
IF 5.175
Times Cited 16
C.elegans tm2482