| RRC ID |
53263
|
| Author |
Truttmann MC, Cruz VE, Guo X, Engert C, Schwartz TU, Ploegh HL.
|
| Title |
The Caenorhabditis elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones.
|
| Journal |
PLoS Genet
|
| Abstract |
Protein AMPylation by Fic domain-containing proteins (Fic proteins) is an ancient and conserved post-translational modification of mostly unexplored significance. Here we characterize the Caenorhabditis elegans Fic protein FIC-1 in vitro and in vivo. FIC-1 is an AMPylase that localizes to the nuclear surface and modifies core histones H2 and H3 as well as heat shock protein 70 family members and translation elongation factors. The three-dimensional structure of FIC-1 is similar to that of its human ortholog, HYPE, with 38% sequence identity. We identify a link between FIC-1-mediated AMPylation and susceptibility to the pathogen Pseudomonas aeruginosa, establishing a connection between AMPylation and innate immunity in C. elegans.
|
| Volume |
12(5)
|
| Pages |
e1006023
|
| Published |
2016-5-1
|
| DOI |
10.1371/journal.pgen.1006023
|
| PII |
PGENETICS-D-15-02363
|
| PMID |
27138431
|
| PMC |
PMC4854385
|
| MeSH |
Animals
Caenorhabditis elegans / chemistry
Caenorhabditis elegans / genetics*
Caenorhabditis elegans / microbiology
Caenorhabditis elegans Proteins / genetics*
Carrier Proteins / chemistry
Carrier Proteins / genetics
Crystallography, X-Ray
DNA-Binding Proteins / chemistry
DNA-Binding Proteins / genetics*
DNA-Binding Proteins / metabolism
HSP70 Heat-Shock Proteins / chemistry
HSP70 Heat-Shock Proteins / genetics
HSP70 Heat-Shock Proteins / metabolism
Heat-Shock Response / genetics
Histones / genetics
Histones / metabolism
Humans
Immunity, Innate / genetics
Membrane Proteins / chemistry
Membrane Proteins / genetics
Nucleotidyltransferases / genetics*
Peptide Chain Elongation, Translational*
Protein Conformation
Pseudomonas aeruginosa / genetics
Pseudomonas aeruginosa / pathogenicity
Recombinant Proteins / chemistry
Recombinant Proteins / genetics*
Recombinant Proteins / metabolism
|
| IF |
5.175
|
| Times Cited |
16
|
| Resource |
| C.elegans |
tm2482 |
