論文 - 詳細
| RRC ID | 53275 |
|---|---|
| 著者 | Crombie TA, Tang L, Choe KP, Julian D. |
| タイトル | Inhibition of the oxidative stress response by heat stress in Caenorhabditis elegans. |
| ジャーナル | J Exp Biol |
| Abstract |
It has long been recognized that simultaneous exposure to heat stress and oxidative stress shows a synergistic interaction that reduces organismal fitness, but relatively little is known about the mechanisms underlying this interaction. We investigated the role of molecular stress responses in driving this synergistic interaction using the nematode Caenorhabditis elegans To induce oxidative stress, we used the pro-oxidant compounds acrylamide, paraquat and juglone. As expected, we found that heat stress and oxidative stress interact synergistically to reduce survival. Compared with exposure to each stressor alone, during simultaneous sublethal exposure to heat stress and oxidative stress the normal induction of key oxidative-stress response (OxSR) genes was generally inhibited, whereas the induction of key heat-shock response (HSR) genes was not. Genetically activating the SKN-1-dependent OxSR increased a marker for protein aggregation and decreased whole-worm survival during heat stress alone, with the latter being independent of HSF-1. In contrast, compared with wild-type worms, inactivating the HSR by HSF-1 knockdown, which would be expected to decrease basal heat shock protein expression, increased survival during oxidative stress alone. Taken together, these data suggest that, in C. elegans, the HSR and OxSR cannot be simultaneously activated to the same extent that each can be activated during a single stressor exposure. We conclude that the observed synergistic reduction in survival during combined exposure to heat stress and oxidative stress is due, at least in part, to inhibition of the OxSR during activation of the HSR. |
| 巻・号 | 219(Pt 14) |
| ページ | 2201-11 |
| 公開日 | 2016-7-15 |
| DOI | 10.1242/jeb.135327 |
| PII | jeb.135327 |
| PMID | 27207646 |
| MeSH | Adaptation, Physiological / drug effects Adaptation, Physiological / genetics Animals Biomarkers / metabolism Caenorhabditis elegans / drug effects Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / metabolism DNA-Binding Proteins / metabolism Environment Gene Expression Regulation / drug effects Genes, Reporter Green Fluorescent Proteins / metabolism Heat-Shock Response / drug effects Heat-Shock Response / genetics Heat-Shock Response / physiology* Oxidants / toxicity Oxidative Stress* / drug effects Oxidative Stress* / genetics Protein Aggregates / drug effects RNA Interference / drug effects Signal Transduction / drug effects Signal Transduction / genetics Survival Analysis Transcription Factors / metabolism |
| IF | 3.179 |
| 引用数 | 7 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1817 |