RRC ID 53284
Author Wang P, Liu H, Wang Y, Liu O, Zhang J, Gleason A, Yang Z, Wang H, Shi A, Grant BD.
Title RAB-10 Promotes EHBP-1 Bridging of Filamentous Actin and Tubular Recycling Endosomes.
Journal PLoS Genet
Abstract EHBP-1 (Ehbp1) is a conserved regulator of endocytic recycling, acting as an effector of small GTPases including RAB-10 (Rab10). Here we present evidence that EHBP-1 associates with tubular endosomal phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] enriched membranes through an N-terminal C2-like (NT-C2) domain, and define residues within the NT-C2 domain that mediate membrane interaction. Furthermore, our results indicate that the EHBP-1 central calponin homology (CH) domain binds to actin microfilaments in a reaction that is stimulated by RAB-10(GTP). Loss of any aspect of this RAB-10/EHBP-1 system in the C. elegans intestinal epithelium leads to retention of basolateral recycling cargo in endosomes that have lost their normal tubular endosomal network (TEN) organization. We propose a mechanism whereby RAB-10 promotes the ability of endosome-bound EHBP-1 to also bind to the actin cytoskeleton, thereby promoting endosomal tubulation.
Volume 12(6)
Pages e1006093
Published 2016-6-1
DOI 10.1371/journal.pgen.1006093
PII PGENETICS-D-15-03079
PMID 27272733
PMC PMC4894640
MeSH Actin Cytoskeleton / metabolism Actins / metabolism* Animals Biological Transport / physiology Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / metabolism* Endocytosis / physiology Endosomes / metabolism* Intestinal Mucosa / metabolism Phosphatidylinositol 4,5-Diphosphate / metabolism Protein Binding / physiology Protein Transport / physiology Vesicular Transport Proteins / metabolism* rab GTP-Binding Proteins / metabolism*
IF 5.224
Resource
C.elegans tm2523 tm1447