| RRC ID |
53284
|
| Author |
Wang P, Liu H, Wang Y, Liu O, Zhang J, Gleason A, Yang Z, Wang H, Shi A, Grant BD.
|
| Title |
RAB-10 Promotes EHBP-1 Bridging of Filamentous Actin and Tubular Recycling Endosomes.
|
| Journal |
PLoS Genet
|
| Abstract |
EHBP-1 (Ehbp1) is a conserved regulator of endocytic recycling, acting as an effector of small GTPases including RAB-10 (Rab10). Here we present evidence that EHBP-1 associates with tubular endosomal phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] enriched membranes through an N-terminal C2-like (NT-C2) domain, and define residues within the NT-C2 domain that mediate membrane interaction. Furthermore, our results indicate that the EHBP-1 central calponin homology (CH) domain binds to actin microfilaments in a reaction that is stimulated by RAB-10(GTP). Loss of any aspect of this RAB-10/EHBP-1 system in the C. elegans intestinal epithelium leads to retention of basolateral recycling cargo in endosomes that have lost their normal tubular endosomal network (TEN) organization. We propose a mechanism whereby RAB-10 promotes the ability of endosome-bound EHBP-1 to also bind to the actin cytoskeleton, thereby promoting endosomal tubulation.
|
| Volume |
12(6)
|
| Pages |
e1006093
|
| Published |
2016-6-1
|
| DOI |
10.1371/journal.pgen.1006093
|
| PII |
PGENETICS-D-15-03079
|
| PMID |
27272733
|
| PMC |
PMC4894640
|
| MeSH |
Actin Cytoskeleton / metabolism
Actins / metabolism*
Animals
Biological Transport / physiology
Caenorhabditis elegans / metabolism
Caenorhabditis elegans Proteins / metabolism*
Endocytosis / physiology
Endosomes / metabolism*
Intestinal Mucosa / metabolism
Phosphatidylinositol 4,5-Diphosphate / metabolism
Protein Binding / physiology
Protein Transport / physiology
Vesicular Transport Proteins / metabolism*
rab GTP-Binding Proteins / metabolism*
|
| IF |
5.175
|
| Resource |
| C.elegans |
tm2523
tm1447 |
