RRC ID 53289
Author Zhou Q, Li H, Li H, Nakagawa A, Lin JL, Lee ES, Harry BL, Skeen-Gaar RR, Suehiro Y, William D, Mitani S, Yuan HS, Kang BH, Xue D.
Title Mitochondrial endonuclease G mediates breakdown of paternal mitochondria upon fertilization.
Journal Science
Abstract Mitochondria are inherited maternally in most animals, but the mechanisms of selective paternal mitochondrial elimination (PME) are unknown. While examining fertilization in Caenorhabditis elegans, we observed that paternal mitochondria rapidly lose their inner membrane integrity. CPS-6, a mitochondrial endonuclease G, serves as a paternal mitochondrial factor that is critical for PME. We found that CPS-6 relocates from the intermembrane space of paternal mitochondria to the matrix after fertilization to degrade mitochondrial DNA. It acts with maternal autophagy and proteasome machineries to promote PME. Loss of cps-6 delays breakdown of mitochondrial inner membranes, autophagosome enclosure of paternal mitochondria, and PME. Delayed removal of paternal mitochondria causes increased embryonic lethality, demonstrating that PME is important for normal animal development. Thus, CPS-6 functions as a paternal mitochondrial degradation factor during animal development.
Volume 353(6297)
Pages 394-9
Published 2016-7-22
DOI 10.1126/science.aaf4777
PII science.aaf4777
PMID 27338704
PMC PMC5469823
MeSH Animals Autophagy Caenorhabditis elegans / embryology* Caenorhabditis elegans / enzymology Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* DNA, Mitochondrial / metabolism* Embryo, Nonmammalian / cytology Embryo, Nonmammalian / enzymology Endodeoxyribonucleases / genetics Endodeoxyribonucleases / metabolism* Fertilization* Male Mitochondria / enzymology* Mitochondrial Membranes / metabolism Mitochondrial Proteins / genetics Mitochondrial Proteins / metabolism* Proteasome Endopeptidase Complex / metabolism Spermatozoa / enzymology Spermatozoa / ultrastructure
IF 41.063
Times Cited 64
C.elegans tm3222 tm2595