論文 - 詳細
| RRC ID | 53393 |
|---|---|
| 著者 | Chen F, Chisholm AD, Jin Y. |
| タイトル | Tissue-specific regulation of alternative polyadenylation represses expression of a neuronal ankyrin isoform in C. elegans epidermal development. |
| ジャーナル | Development |
| Abstract |
Differential mRNA polyadenylation plays an important role in shaping the neuronal transcriptome. In C. elegans, several ankyrin isoforms are produced from the unc-44 locus through alternative polyadenylation. Here, we identify a key role for an intronic polyadenylation site (PAS) in temporal- and tissue-specific regulation of UNC-44/ankyrin isoforms. Removing an intronic PAS results in ectopic expression of the neuronal ankyrin isoform in non-neural tissues. This mis-expression underlies epidermal developmental defects in mutants of the conserved tumor suppressor death-associated protein kinase dapk-1 We have previously reported that the use of this intronic PAS depends on the nuclear polyadenylation factor SYDN-1, which inhibits the RNA polymerase II CTD phosphatase SSUP-72. Consistent with this, loss of sydn-1 blocks ectopic expression of neuronal ankyrin and suppresses epidermal morphology defects of dapk-1 These effects of sydn-1 are mediated by ssup-72 autonomously in the epidermis. We also show that a peptidyl-prolyl isomerase PINN-1 antagonizes SYDN-1 in the spatiotemporal control of neuronal ankyrin isoform. Moreover, the nuclear localization of PINN-1 is altered in dapk-1 mutants. Our data reveal that tissue and stage-specific expression of ankyrin isoforms relies on differential activity of positive and negative regulators of alternative polyadenylation. |
| 巻・号 | 144(4) |
| ページ | 698-707 |
| 公開日 | 2017-2-15 |
| DOI | 10.1242/dev.146001 |
| PII | dev.146001 |
| PMID | 28087624 |
| PMC | PMC5312038 |
| MeSH | Animals Ankyrins / genetics Ankyrins / metabolism* Caenorhabditis elegans / embryology* Caenorhabditis elegans Proteins / metabolism* Cell Nucleus / metabolism Death-Associated Protein Kinases / metabolism Epidermis / embryology* Gene Expression Profiling Gene Expression Regulation, Developmental* Green Fluorescent Proteins / metabolism Introns Mutation Neurons / metabolism* Phenotype Polyadenylation Protein Isoforms RNA, Messenger / metabolism Tissue Distribution |
| IF | 5.611 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2304 tm349 tm2235 |